The Clinical Values Of The Ca2+-binding Protein S100A12in The Acute Cerebral Infarctions And Its Relevant In The Stability Of Atherosclerotic Plaques

Cerebral embolization due to carotid atherosclerosis is a major cause of transientischemic attacks and ischemic stroke. Though it is normally recognized thatinflammation plays a key role in the pathogenesis of atherosclerosis, our understandingof the regulation of this inflammatory process as well as the identification andcharacterization of the different mediators are not complete. The detection of “novel”participants in this complex process could therefore potentially lead to the discovery ofnew biomarkers for this disorder and a new target for therapy. S100A12areCa2+-binding proteins that are predominantly expressed in myeloid-derived cells suchas neutrophils, monocytes, and dendritic cells.They have been implicated in theregulation of a variety of cellular activities including inflammation. In fact, theseproteins are highly expressed in numerous inflammatory conditions such asinflammatory bowel disease, rheumatoid arthritis, and transplant rejection, tumor andthey have also recently been found in murine and human atherosclerotic lesions. Theirmechanisms of action are rather complex involving intracellular and extracellular rolesin modulating calcium signaling, arachidonic acid metabolism, and inflammatoryactivation of leukocytes through innate immune signaling pathways. Despite thiscomplexity, these proteins are thought to be reliable markers of inflammation.Althoughthere are several reports regarding the role of calgranulins in atherosclerosis, few studieshave examined their relationship to plaque stability in patients with atherosclerosis.Inorder to identity the clinical values of the Ca2+-binding protein S100A12expression inthe acute cerebral infarctions and its relevant in the stability of atherosclerotic, serumS100A12and hs-CRP level was tested.ObjectiveAtherosclerosis is a progressive chronic disease accompanied by an inflammatoryresponse. The calcium-binding protein S100A12is involved in many cellular activitiesand pathological processes including inflammation. Based on the different types of ofacute ischemic stroke,S100A12expression in peripheral blood monocytes (PBMC) were detected in patients and study the clinical significance in acue cerebralinfraction.We purposed to explore the correlation between plasma S100A12levels andstability of atherosclerosis plaque.Methods64consecutive patients with acute ischemic stroke were recruited. They areclassified with SSS-TOAST as large artery atherosclerosis (LAA) type31cases,small-artery occlusion (SAO) type21cases and cardioaortic embolism (CAE) type12cases.12patients with carotid artery stenosis (CAS) without stroke were recruited aspure carotid artery stenosis and10normal people as normal control. Patients with acutecerebral infarction were scored with NIHSS to comment their neurological function.ThemRNA levels of plasma S100A12and hs-CRP were measured by enzyme linkedimmunosorbent assay. Results from different groups were analyzed.Results1. Serum hs-CRP levels in patients with artery atherosclotic infarctions were higherthan pure carotid artery stenosis (P<0.01).2. The Plasma levels of S100A12levelexpression of atherosclerosis in patients with acute cerebral infarction (serum S100A12level higher than without atherosclerosis (P<0.01), the diagnostic sensitivity andspecificity of atherosclerosis, higher than the hs-CRP serum (P<0.05). Arteryatherosclerotic cerebral infarction was higher than other types of cerebral infarction (P <0.01), the diagnosis of the sensitivity of the artery atherosclerotic cerebral infarction ishigher than the hs-CRP (P<0.05).3. There was positive relevance between the levels ofserum S100A12and NIHSS scores (r=0.680, P<0.01) and their correlation was superiorto hs-CRP (r=0.823, P<0.01)ConclusionIn the atherosclerotic cerebral infarction patients serum hs-CRP level is higher,shows that it is associated with plaque stability; Serum S100A12expression level isobviously higher, prove S100A12is associated with atherosclerotic cerebral infarction,Compared with serum hs-CRP, in judging atherosclerosis, plaque stability and arteryatherosclerotic cerebral infarction has higher. By NIHSS score in patients with acuteischemic stroke, serum S100A12level has positive phase correlation with NIHSS score,and the correlation is higher than the hs-CRP serum, proved S100A12gross judgementcondition degree of a index of acute ischemic stroke. Our findings support a linkbetween S100A12and atherogenesis and suggest that S100A12might be a newbiomarker of plaque stability.