Study On The Intervention Effect And It’s Mechanism Of Huayujiedufa On Brain Injury And High Coagulation State In MCAO Rats

Objective:To observe the effect of Huayujiedufa on thrombin mRNA, thrombus biomarkers, the volume of cerebral infarction by establishing middle cerebral artery occlusion model in rats, and to investigate the protective effect and its mechanism of Huayujiedufa.Methods:All84male SD rats were randomly divided into sham-operated group,Model group, Huoxuehuayu group, Qingrejiedu group, Huayujiedu group, PDTC group, Argatroban group.Rat model of middle cerebral artery occlusion was established first, and the corresponding drugs were given at1hour before operation and12hours after operation, samples were taken for medical analysis24hours later after operation.PCR method was used to detect the expression levels of thrombin mRNA in rat brain;Elisa mehod was used to detect the levels of TAT、F1+2、 D-dimer、 vWF in rat plasma;TTC staining was used to observe the volume of cerebral infarction; Electron microscope was used to observe the pathological changes.Results:1.The expression level of thrombin mRNA in model group was significantly higher than that in sham operation group(P<0.05); Compared with the model group, the expression levels in treatment groups were significantly decreased (P<0.05); Compared with all the treatment groups, The expression level of thrombin mRNA in Argatroban group and Huayujiedufa group werer the lowest (P<0.05); PDTC group was in the second (P<0.05);Qingrejiedu group and Huoxuehuayu group were the highest (P<0.05).2.Compared with the sham operation group, the contents of TAT in model group and treatment groups increased significantly (P<0.05); Compared with the model group, the content of TAT in the treatment groups decreased (P<0.05);Compared with all the treatment groups, the content of TAT in PDTC group was the highest (P<0.05); Huayujiedufa group and Qingrejiedu group were in the second (P<0.05);Argatroban group and Huoxuehuayu group were the lowest (P<0.05).3.Compared with the sham operation group, the contents of F1+2in model group and the treatment groups increased significantly (P<0.05); Compared with the model group, the contents of F1+2in the treatment groups decreased (P<0.05); Compared with all the treatment groups, the content of F1+2in Argatroban group was lower than that in PDTC group, Huayujiedufa group, and Huoxuehuayu group (P<0.05); The content of F1+2in Hayujiedufa group was lower than that in PDTC group (P<0.05).4.The content of D-dimer in model group was significantly higher than that in the sham operation group (P<0.05);Compared with the model group, the content of D-dimer in Argatroban group decreased (P<0.05), and increased in Qingrejiedu group (P<0.05); There was no significant difference between Huoxuehuayu group, Huayujiedufa group, PDTC group and model group (P>0.05).5.The content of vWF in model group was significantly higher than that in the sham operation group (P<0.05); The contents of vWF in the treatment groups decreased compared with the model group (P<0.05):Compared with all the treatment groups, the content of vWF in Huoxuehuayu group were higher than that in PDTC group and Qingrejiedu group (P<0.05);There was no significant difference between Huoxuehuayu group, Argatroban group and Hayujiedufa group (P>0.05).6. Except the sham operation group, model group and the treatment groups showed focal infarction; Compared with the model group, the infarction volume in the treatment groups were significantly reduced (P<0.05);Compared with all the treatment groups, the infarction volume in Argatroban group was the smallest, Huayujiedufa group was in the second, Qingrejiedu group was in the third, Huoxuehuayu group and PDTC group were in the last.7.There was synergistic effect in compatibility Huoxuehuafa and Qingrejiedufa. Each method could reduced the levels of thrombin mRNA, TAT, F1+2,and vWF, when the two methods were used at the same time there was statistical significance in the interaction (P<0.05).8. The content of TAT, F1+2, vWF in rats plasma showed a positive linear correlation with the expression of thrombin mRNA(P<0.05); The infarction volume also showed a positive linear correlation with the expression of thrombin mRNA (P<0.05).9. Electron microscopy showed that the structure of the nerve cells were clear and regular in the sham operation group; While in the model group the microstructure is blurry:nerve cells were irregular in shape, the nuclear membranes were uneven, nucleus dissolved, cell organelles were swelling, myelin sheathes were twisted, separated or fractured; The changes of each treatment group were less than that of model group. Conclusion:There were significant brain tissue damage and thrombin mRNA expression in middle cerebral artery occlusion model rats. The blood is in the hypercoagulable state, it showed coagulation and fibrinolysis hyperfunction, and enhanced platelet activation; Huoxuehuayufa, Qingrejiedufa, Huayujiedufa all could improve this state. Huoxuehuayufa focused mainly on improving the coagulation and fibrinolysis function, and Qingrejiedufa mainly focused on improving the platelet and endothelial function, while Huayujiedufa had effect on both, and it was synergistic effect.