The Effect Of Bushen Huoxue Tonglin Decoction On Ki-67 And Caspase-3 Of Model Rats With Benign Prostatic Hyperplasia(BPH)

Objective:The project intends to angle from apoptosis and proliferation, to observe the changes in the prostate tissue of rats in each experimental group, Ki-67 and Caspase-3 in rats to evaluate Bushenhuoxue Tonglin prostate hyperplasia model Ki-67 and Caspase- 3 in explore Bushenhuoxue Tonglin treatment of benign prostatic hyperplasia mechanism of action, disease-oriented medical treatment provides a scientific basis.Methods:Clean grade male SD rats weighing 230-260 g, 3 months old. After weighing numbers randomly divided into six groups.Rats fed the first week, let it adapt to the environment began modeling. 6 Group anesthesia are used by 10% of chloral hydrate(3.5mg / kg) by intraperitoneal injection of the way, the surgery should be strictly in accordance with the requirements of animal experiments aseptic, cut intraperitoneal double their testes removed; sham surgery cut only after intraperitoneal anesthesia group, not castrated to only testis in the free state. 7 Angels were observed feeding its wound healing, sham surgery group were not injected testosterone propionate, give 15 ml / kg of saline, and the remaining five groups according to the modeling method [1] modeling, modeling and administration at the same time, the model group give 15 ml / kg of saline, Proscar group received 0.8mg / kg finasteride tablets solution(equivalent to 10 times the clinical dosage), BHD Tonglin low dosage group were given medicine 7.5g / kg(quite to 5 times the clinical dosage), BHD Tonglin prescription medicine dose group were given 15 g / kg(equivalent to clinical use. 10 times the dose), high dose BHD Tonglin party. amount group were given medicine 22.5g / kg(equivalent to 15 times the clinical dosage). These drugs are diluted with physiological saline in accordance with 15 ml / kg using standard gavage, once a day, rats were weighed weekly to adjust the dosage for consecutive 30 days. The next day after the last administration, the rats were sacrificed by cervical dislocation, cut prostate tissue to be tested and set aside.The expression by RT-PCR detection of apoptosis regulatory factor Caspase-3m RNA; the use of technology to detect immune-free group of proliferating cellnuclear antigen Ki-67 expression and Caspase-3’s.Result:The expression of Caspase-3m RNA in the treatment groups was increased compared with model group(P<0.05). The expression of Caspase-3 in high dose Bushen Huoxue Tonglin Decoction group was increased compared with Proscar group and low dose Bushen Huoxue Tonglin Decoction group(P<0.05).Compared withthe model group, the prostatic index and the expression of Ki-67 in treatment groups was decreased(P < 0.05). Compared with Proscargroup and low-dose BSHXTLF group, the expression of Ki-67 in the middle-and high-dose Bushen Huoxue Tonglin Decoction groupwas decreased(P < 0.05). Compared with the middle-dose Bushen Huoxue Tonglin Decoction group, and the high-dose group compared with the low-dose group, the Ki-67 expression decreased(P < 0.05).Conclusion: Bushenhuoxue Tonglin Decoction can reduce the expression of benign prostatic hyperplasia Ki-67 to reduce the proliferation of prostate tissue cells; increased expression of benign prostatic hyperplasia Caspase-3, to speed up the prostate tissue apoptosis. This may be Bushenhuoxue Tonglin party intervention mechanisms of benign prostatic hyperplasia.