Study On The Anti-obesity Activity And Mechanism Of COST Particles

Obesity is a chronic disease due to the imbalance of the body’s energy metabolism. It seriously harms human health. At present, the weight loss drug market is influenced by the factors such as curative effect, side effect, development cost and so on. The number of drug losing weight is rare. At the same time, anti-obesity drug has been due to safety problems concern, leading to the diet pills on the market mostly approved only for body mass index(BMI) is more than or equal to 30 patients(obese), or BMI greater than or equal to 27(overweight) and at least associated with a body weight related diseases, such as type 2 diabetes or high cholesterol, hypertension, dyslipidemia patients. Therefore, the research and development of the safety, effective, quality controllable anti-obesity drug has become popular.Chitosan oligosaccharides(COS) is obtained by enzymatic hydrolysis or chemical degradation of chitosan(chitosan, CTS). The research showed that it had the higher activity of reducing weight, and had the characteristic of low toxicity and less adverse reaction. This is compatible with the characteristics of chronic medication for obesity, which has been studied in many fields.The research group has systematically studied the COS weight loss activity. Compared with CTS, COS had better solubility and higher bioavailability. COS showed a stronger effect of weight loss, at the same time, the experimental results showed that the relative other molecular weight COS, the average molecular weight of less than COS 1000Da(COST) weight loss is the most significant. This paper studies the mechanism of weight loss of COST from two different mechanisms.The first mechanism of the mechanism of differentiation into mature adipocytes by 3T3-L1 before the differentiation of adipocyte differentiation and the process of increasing. By COST, the effects of COST on adipocyte differentiation and intracellular lipid accumulation, and the effects of PPAR and CEB/P expression on the major related genes were studied. The results showed that the cellular level, cost can significantly inhibited 3T3-L1 pre adipocytes into adipogenic differentiation and lipid accumulation process, reduce the rate of differentiation of pre-adipocytes, reduce the mature adipocyte triacylglycerol accumulation. COST can inhibit the expression of CEB/P and m RNA PPAR, inhibit the differentiation and lipid accumulation of fat cells and inhibit the effect of obesity. The results of this study and research group preliminary in vivo experiments results are consistent. It is proved that the cost may inhibit adipocyte differentiation and lipid accumulation, decrease fat pad weight and body fat than the way to achieve the purpose of weight loss.The second mechanism, the early research that chitosan load capsaicin microspheres(CCMS) can reduce the expression of leptin m RNA in obese rats serum leptin level and tissue. The effect of COST on leptin in rats was studied in this paper. The model of nutritional obesity in SD rats(with leptin resistance) was established. The contents of serum lipids and leptin in serum were determined by using reagent kit, and the expression of Receptor Leptin(OB-Rb) and STAT3 in brain was detected by blotting Western. So as to determine the phenomenon of leptin resistance in obese rats, COST can decrease serum leptin level and increase the expression of leptin receptor related proteins in brain and alleviate the phenomenon of leptin resistance. Thus, COST can improve the leptin receptor sensitivity in rats and improve the obesity caused by leptin resistance.Thus, this paper concludes that COST may be the conclusion that the two mechanisms of obesity are treated by inhibiting the increase of fat cells and leptin resistance.