Study On Chemical Composition Of Fermentation Products From Pleurotus Eryngii Var. Tuoliensis

Fermentation products play an important role in human life because many fermentation products show a variety of biological activity such as antibacterial antitumor,anti-inflammatory and so on. Edible medicinal fungus is big natural source of the Fermentation products. The Fermentation products in them may h ave unique structures and biological activity that may be lead compounds in th e researching and development of new drugs. This may have important referen ce value to deal with various complex diseases of people.Submerged fermentation is a kind of efficient culture methods for enrichm ent of fermentation products of fungi.we can obtain a large number of ferment ation products of fungi and mycelium. Solid-state fermentation is also an effect ive method to produce many fermentation products which different from subme rged fermentation.Pleurotus eryngii var. tuoliensis is a kind of precious edible and medicinal mushroom from Xinjiang. This study obtain the extract from solid and liquor f ermentation by ethyl acetate, and separate and purify the extract, text the cytot oxicity of the compounds against the MCF-7,S180 and B16 cell-line.1 Through liquor fermentation of P. eryngii var. tuoliensis, with the OD600 value of the fermented liquor and mycelial dry weight growth the curve dra wing of P. eryngii var. tuoliensis confirm the time is 6.5d. The 50 L fermentati on tank is used for the liquor culture. On the condition of liquid volume(60%),inoculation(5%), temperature(26℃), PH(natural), tank pressure(0.05Mpa), ventil ation(0.6v/v.m), rotation rate(160r/min). The content of reducing sugar first incr ease and then decrease, finally reach the minimum value; the content of protei n in contrast with the content of reducing sugar; and the content of extracell ular polysaccharide with the extension of the time reach the highest value. Obt ain dry mycelium 356 g, the yield is 1.12g/100 mL. Dry crude extracellular poly saccharide 220 g, the yield is 0.6g/100 m L. Solid state fermentation of the P. er yngii var. tuoliensis with the medium of rice and water, ethyl acetate extractio n, get P. eryngii var. tuoliensis fermentation solid extract 86 g. 2 Modern separation methods such as thin layer chromatography, silica gel- column chromatography, HPLC etc, and spectral techniques including 13C-NMR, 1H-NMR, DEPT were employed for isolation and identification of the compounds. Structures of 11 compounds were elucidated by detailed spectroscopic analysis and compared with literature data. They are cyclo-(L-Phe-L-pro)(1), α-D-glucose(2), Lauric acid(3), 10,12-dihydroxy-8(E)-octadecenoic acid(4), dibutyl ph-thalate(6), diisobutyl phthalate(7), neo-inositol(8),β-D-glucose(9). All of the compounds were the first time isolated from the fermentation product of P. eryngii var. tuoliensis.3. Vitro antitumor experiment(determined by MTT method) was carried on for the part of the compounds and fermentation extract.The results of the anti tumor vitro experiment showed that compound 4 had the best activity to the MCF-7, B16 and S180 cellline. Its corresponding IC50 values were 0.059mg/m1, 0.063mg/ml and 0.117mg/ml.