Signal Regulatory Mechanisms Of INOS In MG63 Osteosarcoma Cells

Objective: Discussion of MG63 human osteosarcoma cells VEGF-C / VEGFR-3 signaling pathway activation or inhibition, MG63 cells release NO, i NOS expression intensity(i NOS protein and i NOSm RNA quantitative), statistical analysis MG63 cells VEGF-C / VEGFR-3 signaling pathway activation of i NOS.Methods: In order to observe the relationship between MG63 cells VEGF-C / VEGFR-3 signaling pathway in the activation or inhibition of i NOS activity, and there were 4 group for our research:(1) MG63 cell line groups: control;(2) MG63 cell line + VEGF-C Group: Training yl added VEGF-C( concentration of 100 ng / m L);(3) MG63 cell line + Ag group: culture medium was added Ag(at a concentration of 5μM);(4) MG63 cell line + VEGF-C + MAZ-51 group: culture medium was added VEGF-C and MAZ-51.(1) NO reduction method for the determination of the concentration of NO in the culture medium.(2) Westen-blot assay cells in each group of i NOS protein levels.(3) RT-PCR assay expression levels of MG-63 cells of i NOS m RNA.(4) Immunofluorescence to detect the expression of i NOS expressionResults:(1) MAZ-51 for VEGFR-3 inhibition, the expression of i NOS were reduced, NO emission reduction.(2) Ag for i NOS inhibition, reduced expression of i NOS, NO emission reduction.(3) VEGF-C / VEGFR-3 for the MG-63 osteosarcoma cells i NOS expression has significant role in promoting.Conclusion: The expression of i NOS in osteosarcoma was increased in promoting VEGF-C / VEGFR-3, and promote the release of NO.