Evaluation Of Ginsenoside Contents In Ginseng And Its Preparation

Ginseng is a famous Chinese herbal medicine, which has been used frequently in traditional Chinese medicine for thousands of years. The kinds and contents of ginsenoside are important standards of ginseng in the quality evaluation. But the study of simultaneous determination of multiple ginsenosides in ginseng and its preparation by HPLC has not yet been reported. A simple and accurate HPLC for determining the contents of 16 ginsenosides from ginseng and its preparation was established in the study. And the study analyzed the contents of 16 ginsenosides in white ginseng, red giseng, fresh-keeping ginseng and ginseng preparation of different manufacturers(Renshen Jianpi Pill, Renshen Guipi Pill, Renshen Yangrong Pill, Wuji Baifeng Pill and Shengmai Yin) by HPLC. At the same time, the contents of 9 ginsenosides were compared in one-year-old farmland fresh ginseng and American ginseng in the study. The study provides scientific testing method and theoretical basis for the quality control of ginseng and its preparation. The conclusions of this study are as follows:First, a simple and accurate HPLC for determining simultaneously the contents of 16 ginsenosides(Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, Rh2, protopanaxatriol, compound K, protopanaxadiol)from ginseng and its preparation was established in this study. The method is simple, fast and accurate, and could be applied to the quality control of ginseng and its preparation. The method could identify whether ginseng preparation contained ginseng stems and leaves or not.Second, the kinds and contents of ginsenoside were different among different manufacturers of the same ginseng preparation(P<0.05). In addition, ginsenosides like F1, F2, Rh2 were detected in ginseng preparation which could not be found in ginseng. Amphibolic efficacy of ginseng preparation is caused by its unstable efficacy material basis. It has great significance to establish the quality standard of ginseng preparations.Third, the white ginseng contained 12 ginsenosides like Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, Rd, protopanaxatriol, Rh2, protopanaxadiol, red ginseng contained 12 ginsenosides like Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, Rd, Rg3, compound K, protopanaxadiol, and fresh-keeping ginseng contained 10 ginsenosides like Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, Rd, protopanaxadiol. The contents of ginsenosides in white ginseng were higher than that in red ginseng and fresh-keeping ginseng, the contents of ginsenosides in red ginseng were higher than that in fresh-keeping ginseng. And ginsenosides Rg3 and compound K were determined in red ginseng.Last, one-year-old farmland ginseng contained 9 ginsenosides like Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd, and American ginseng contained 7 ginsenosides such as Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd. The contents of ginsenosides in American ginseng were higher than that in farmland ginseng except for Rb2. The contents of ginsenosides in farmland ginseng and American ginseng met the quality standards of Chinese Pharmacopoeia.