Effects Of L-Ornithine Alpha-Ketoglutarate Dihydrate On Intestinal Immune Function In Severe Intrabdominal Infection Rats

Objective:To investigate the effects of immune nutrients(OKG)enriched enteral nutrition on intestinal immune function in severe intrabdominal infection rats. Methods:After sepsis rats was induced by cecal ligation and puncture(CLP),20 male SD rats were randomly divided into normal enteral nutrition group(group EN,N=10) and enteral immune nutrition group(group EIN,N=10).EN group was fed with normal enteral nutrition liquid. EIN group was given OKG-enriched enteral nutrition. The two groups rats received isocaloric, isonitrogenous,and isovolumic enteral nutrition. The relative proportions of energy in both groups were 32% carbohydrate,50% fat and 18% protein. The energy requirement of each rat was calculated according to 882KJ?kg-1?d-1.The rats were feed in the early postoperative period. Continuous feeding for 7 days,all rats were killed. Collect whole section intestine and count total cell numbers in peyer’s patches. Use flow cytometry to measure the numbers of T and B cells in peyer’s patches. Changes in the morphology of intestinal mucosa was observed by electron microscopy. Measure the jejunum villus height. Results:EIN group compared with the normal enteral nutrition group,the numbers of total cells 、T and B cells in peyer’s patches were significantly higher. The difference was statistical significance(P<0.05). Conclusions:OKG-enriched enteral nutrition can increase the numbers of total cells 、T and B cells in peyer’s patches in severe intrabdominal infection rats, improve the structure of intestinal epithelial tissure. Improve the rats intestine immune function.