The Study Of MiR-21 Expression And Its Mechanisms Of Cell Proliferation And Apoptosis In Laryngeal Squamous Carcinoma

BackgroundLaryngeal cancer in head and neck tumors was 13.9%, second only to nasopharyngeal and nasal cancer, living in third place, and in recent years its incidence increasing year by year. A large number of scholars have found that the incidence of laryngeal cancer has involved variety oncogenes and tumor suppressor genes in the process of multiple stages of cascade, its occurrence and development may be due to oncogene actived, deletion of tumor suppressor gene or had mutants caused, and then teaded to excessive proliferation of tumor cells; In addition it may also be due to the inhibition of apoptosis,and then leaded to the cancer cell survival. But the regulator of cell proliferation and/or apoptotic were changed, it could lead to the ancer.ObjectiveThis study analyzed miR-21 and Bcl-2 expression and the significance of them in laryngeal squamous carcinoma, and investigate the effect of miR-21 expression to Bcl-2. We also detected the affect of miR-21 to Hep-2 cells about cell proliferation, apoptosis and invasion, designed to understand the occurrence and development of laryngeal squamous carcinoma, and to provide a reliable basis for the clinical diagnosis and targeted therapy of laryngeal squamous carcinoma.Methods(1) We detected the expression of miR21 and Bcl-2 mRNA by qPCR in o laryngeal squamous carcinoma tissues and corresponding normal tissue; detected the expression of Bcl-2 protein by western blot; analysis the correlation of Bcl-2 protein between clinicopathological parameters.(2) miR-21 inhibitor was transfected into Hep-2 cells; we detected the multiplication efficiency of Hep-2 cells by MTT assay. Flow cytometry was used to detect the poptosis in 0 h,24 h,48 h,72 h,96 h after transfection; we also detected the neoplasm invasion after transfection.Results(1) The expression of miR-21 and Bcl-2 mRNA in laryngeal squamous carcinoma was significantly higher than its expression in control group; the expression of Bcl-2 protein in laryngeal squamous carcinoma was significantly higher than its expression in control group; Bcl-2 protein is closely related to clinical stage, lymph node metastasis, and tumor differentiation.(2) After transfection of miR-21 inhibitor, the expression of Bcl-2 protein was significantly decreased; the proliferation and invasion ability of Hep-2 cell were significantly reduced, and the apoptosis was significantly increased.ConclusionsmiR-21 may increase the expression of Bcl-2 to promote the cell proliferation and invasion, and inhibiting cell apoptosis in laryngeal squamous carcinoma, hintting that miR-21 may be an oncogene by involving in the occurrence and development of laryngeal squamous cell carcinoma, which may be also a novel tumor marker for clinical diagnosis, prognosis and targeted therapy.