The Effect Of Sevoflurane Preconditioning On Pulmonary Matrix Metalloproteinases 9 MRNA During Partial Hepatic Ischemia/Reperfusion Lung Injury In Rat

Objective:To observe the intervention of sevoflurane pretreatment on pulmonary matrix metalloproteinases(mmp-9) mRNA caused by rat liver ischemia reperfusion and to explore its mechanism. Methods:fifty-four male Wister rats weighing 270-330 g were randomly divided into 3 groups(n=18 each): sham operation group(group S): the hilum of liver was isolated but not clamped; partial hepatic ischemia-reperfusion(I/R): the left and median lobe of liver was occluded for 30 min followed by reperfusion; sevoflurane preconditioning group(group Sev): 2.1% sevoflurane was inhaled for 30 min.and partial heptic I/R was induced after the end of inhalation in group Sev. Six rats in each group were chosen at 30,60 and 120 min of reperfusion and sacrificed,the serum tumor necrosis factor-α were detected,and lungs were removed for microscope examination of pathologic changes which were scored and for determination of wet/dry lung weight(W/D)ratio,MPO activity(by colorimetrie method)and the expression of MMP-9 mRNA(by RT-PCR).Results:Compared with group S, the serum tumor necrosis factor-α,the W/D ratio,MPO activity and expression of MMP-9 mRNA were significantly increased in groups I/R and Sev(P<0.05).Compared with group I/R, the serum tumor necrosis factor-α,the W/D ratio,MPO activity and expression of MMP-9 mRNA were significantly decreased in group Sev(P<0.05).Conclusion: Severe acute lung injury after partial hepatic I/R in rats is attenuated by sevoflurane.An inhibition of MMP-9 mRNA expression in lung tissues may be one of the mechanisms for that.