| Objective Hydroxy safflower yellow A is now commonly used in clinical medicine, is extracted from the petals of Carthamus tinctorius L, chalcone compounds, is allowed the use of edible natural pigment by China, have been included in the GB2760-1996, state-level new drugs,mainly used in the treatment of cardiovascular and cerebrovascular diseases. The study found that safflower yellow A has a variety of pharmacological effects on inhibiting platelet aggregation, expanding coronary artery, improving cardiac and cerebral ischemia, scavenging oxygen free radicals, anti inflammation, inhibiting immunity and promoting the value of vascular endothelial cells. Based on the preliminary results of these studies, this paper intends to determination of hydroxy safflower yellow A(HSYA) can reduce the skin graft necrosis, inhibited the stimulation of the innate immune system so as to improve the syngeneic mice trunk skin graft from the preserved after transplant survival rate and survival quality, thereby inducing immune tolerance of. The new composition of the organ preservation solution was developed, and the experimental and theoretical basis for the improvement of preservation method was established. In order to further clarify the mechanism and application of the long-term survival and difficult to establish the mechanism and the application of transplantation tolerance.Method: Establishment of skin transplantation model in mice, BALB/c(H-2d) female mice were randomly divided into 6 groups, respectively, did not give medicine group, only intraperitoneal injection group, 250μg/ml concentration group, 2500μg/ml in the high concentration group, 250μg/ml concentration in the combined treatment group, 25μg/ ml high concentration combined medication group, according to the different preservation time will were randomly divided in six groups for preservation of 0, 3, 6hours, the three subgroups, each subgroup 5 animal. 7 days later, the pathological changes of skin graft were observed and the changes of the area and color of skin graft in 7-13 days after operation were evaluated.result: 1.The survival area score(1) Immediately transplantation(interval 0h) group, on 7, 9, 11 and 13 days, mice survival area of change scores was significantly higher than that in the interval 3, 6h all the components of the living area score(P < 0.05);(2) At intervals of 3 h all groups, did not give medicine group and only intraperitoneal injection group score lowest; Middle concentration group, high concentration group and high concentration combined medication group score trends similar, but score in middle concentration group was significantly higher than that in Concentration group and high concentration of the combined medication group(P < 0.05); Middle concentration combined medication group scored the highest in 7-13 days score less volatility, relatively stable and intervals of 3 h and 6 h interval with the change of the number of days had a significant difference in each segment(P < 0.05).(3) At intervals of 6h all groups, to medicine group and only intraperitoneal injection group, the lowest score, in 7-13 days volatility is very small, almost no change; combination of concentration and medium concentration group score was the highest, but in 7-13 days appear significantly decreased; exactly the same score of high concentration group and the high concentration of the combined group, the two lines overlap, the two groups of survival area score in 7-13 days appear slow rise, not fluctuations. 2.Color change score results(1) In the 0h group, the color score had no change in 7-9 days, and 9 days later, there was a significant rise, the color change score was significantly higher than the interval 3, 6h all groups of color change score(P<0.05);(2) At intervals of 3h all groups, the combination group color change score was the highest, the concentration at the time of the four color change score less volatility, thetrend for slightly; middle concentration group, the color change score in 7-11 days fell, but in the 11-13 days has increased significantly not; for the medicine group and the lowest score only intraperitoneal injection group, and the scores have experienced a significant decline in 7-13 days; high concentration and high concentration of drug combination group were relatively close, and in the middle of the 6 group, the 2 groups score when 7-11 days appeared to rise slightly, however in 13 days of decline; with the changes in the color number score was significantly different between 3H and 6h interval(P<0.05);(3)At intervals of 6h all groups, not to the drug group 7-13 days of the lowest score, the concentration of the combined group and only the intraperitoneal injection group, the score is the same, both in the 7-9 days, after the emergence of a sustained decline. And the score of the combined drug group was significantly higher than that of the intraperitoneal injection group. In the concentration group continued to decline in 7-13 days. High concentration group appeared to rise in 7-11 days, but there was a significant decline in the 13 day; the high concentration of the combined drug group in 7-13 days of small fluctuations. 3, gross specimen and pathological section observation results(1) From the gross observation, these drug concentration combined after administration of transplanted skin graft survival best quality; concentration of immersion time, 13 days after skin graft can slow recovery; only intraperitoneal injection to drug and non drug skin graft can not survive, after about 13 days skin graft contracture necrosis.(2) The pathological sections showed that the concentrations of combined drug group were significantly less than those in the non drug group, the degree of edema, necrosis, vascular proliferation, vascular and interstitial inflammatory infiltration were significantly reduced.Conclusion:(1) Syngeneic mice skin transplantation, skin graft from the body immediately transplantation(interval 0h), plant survival quality best, the survival rate is almost100%.(2) In several groups of experimental drug, in concentrations of HSYA soak 3 hours combined with daily intraperitoneal injection after administration, skin graft survival quality the best, and in 7-13 days fluctuations do not, relatively stable; concentration combined to dosing interval 6 hour group score although less than an interval of 3 hours group, but significantly higher than that of interval 6 hours of other groups; that HSYA solution at concentration of combined administration of the inflammatory response after skin graft may inhibit and prolong skin graft survival time, and can promote the skin graft survival quality. |
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