To Analyze The Maternal Exposure To Polycyclic Aromatic Hydrocarbons And Impact On Neonatal Health And Association With Metabolic Enzyme Gene Polymorphism

Objective: To analyze the PAHs(Polycyclic Aromatic Hydrocarbons, called PAHs)exposure scenarios in maternal and the impact on neonatal immune system in Urumqi, to clarify the correlation on gene polymorphisms and maternal exposure to PAHs. Method: 1.To select 315 pairs of maternal and neonatal in heating and non-heating period as the object, to carry epidemiological investigation and biological sample collection. 2. To use the enzymatic hydrolysis-HPLC-mass spectrometry to detecte the 1-OH-P concentrations in maternal urinary,used enzyme-linked immunosorbent assay to detect aryl hydrocarbon receptor(AhR),used immune nephelometry to detect IgA, IgG, IgM concentrations in maternal body. Evaluated the effect on PAHs exposure to neonatal immune system. 3. To genotype the single nucleotide polymorphisms(single nucleotide polymorphism,SNP) of CYP1A1 Msp1 sites,GSTP1 I105 V sites,GSTT1 and GSTM1 gene,analysed the relevance of gene polymorphism and maternal exposure to PAHs,reveal gene- gene and gene-environment interaction.Results: 1. Concentrations of 1-OH-P in maternal were(0.42± 0.40) μg/mmol Cr in urinary, and AhR in maternal and neonatal were(0.30±0.28)ng/mL and( 0.15±0.12) ng/mL.The differences of the AhR concentration in maternal and neonatal body were statistically significant(P<0.05). 2. Differences of 1-OH-P concentrations in maternal and AhR concentration in maternal and neonatal body in heating period and the non-heating period were statistically significant(P<0.05). 3.Education degree of maternal increase one lever, 1-OH-P concentrations decrease0.253 degree. Cooking tools of maternal increase one lever, 1-OH-P concentrations increase0.187 degree.H ome address from the road had positive correlation with AhR concentrations in maternal body(r=0.185,P<0.05). 4. Maternal exposure to PAHs and IgA,IgG, IgM concentration in their parent and offspring was negatively correlated, and maternal exposure to PAHs and IgM concentration and their newborns IgM, IgG concentrations were statistically significant(P<0.05). 5. After controling another effect infectors, Maternal higher 1-OH-P exposure state had a relation to CYP1A1 Msp1 locus genotype(C C), gene- gene interactions of GSTM1 and GSTP1, gene- environment interactions of GSTT1 and passive smokers during pregnancy(P<0.05).GSTP1 I105 V locus genotype(GG) and GSTT1 gene deletion(-) in newborn were effected by higher1-OH-P exposure state in maternal, Conclusion: 1.PAHs exposure will cause different degrees of damage to the immune system to parent and progeny, 2. Home address from the road and cooking tools were risk factor s.3.Maternal exposure higher PAHs levels had relationship with its own CYP1A1 gene, gene- gene interactions of GSTM1 and GSTP1,gene- environment interaction of GSTT1 and passive smoking. 4. newborns GSTP1 and GSTT1 genes mutations were affected by PAHs exposure scenarios in maternal.