Study On Hypolipidemic, Antioxidative Effect And Effective Components In Camellia Japonica L.

In this paper, the petals and leaves of Camellia japonica L. were used as the test material and extracted by ethanol(95%). Extracts was partitioned by petroleum ether, chloroform, ethyl acetate and saturate n-butanol after dissolved in water, and then concentrated and evaporated to afford five fractions. We studied the hypolipidemic, antioxidative effect and effective components of them. The hypolipidemic and antioxidant activity of the five extracts were judged by the bile salt-binding capacity and the removing ratio of DPPH·, ·OH and reducing power, respectively. After correlation analysis, there is a significant positive correlation between the hypolipidemic, antioxidative effect and the content of polyphenol, flavonoids; The result of LC-MS analysis indicated that the catechin and epicatechin might be the major active ingredients to hypolipidemic and antioxidant in Camellia japonica L.. The major results are as follows:1. The extracts yield are as follows:the extraction yield with solvent is different, we used ethyl acetate, n-butanol, petroleum ether, chloroform and distilled water as solvents, and achieved 1.210%,1.701%,0.790%,0.187%,0.330% from petals respectively. And 6.058%, 6.631%,0.362%,1.733%,1.904% from leaves separately.2. It was found that the stability and repeatability of UV method is favorable with the R2 of the standard curves were 0.9953 for sodium taurocholate(STC),0.9969 for glychocholic acid sodium salts(SGC),0.9989 for sodium cholate(SC). The bile salt-binding capacity of petroleum ether, chloroform, ethylacetate, n-butanol and distilled water extracts were compared with the main components of the hypolipidemic drugs under the vitro simulation of human gastric digestive environment. The result showed that the extract of petals is superior than leaves in lipid-lowering, ethyl acetate and n-butanol extracts exhibiting the greatest binding capability in petals. The ethyl acetate extract showed the 84.44% binding capacity for STC, SGC 60.99 and SC 96.95% as well as n-butanol extract showed the 71.33% binding capacity for STC,54.14% for SGC and 70.29% for SC.3. The antioxidant effect of Camellia japonica L. was judged by scavenging DPPH· and ·OH capability and reducing power. The results showed that ethyl acetate and n-butanol extracts of petals have a stronger free radical scavenging ability, and exhibiting a good antioxidant activity in vitro. In the concentration range test, the clearance ratio increased with increasing of concentration to a dose-effect relationship. The n-butanol and ethyl acetate extract from petals is the strongest in scavenging DPPH· capacity, when the sample concentration reached to 200 μg/mL, the scavenging DPPH· capacity of n-butanol and ethyl acetate extracts of petals up to 93.31% and 83.71%, respectively; the scavenging DPPH· capacity of n-butanol and ethyl acetate extracts of leaves up to 76.61% and 61.01%, separately. We also found that the extracts scavenging ·OH capacity is greater than Vc in low concentration, and less than Vc in high concentration, and the clearance ratio rise slowly with the concentration increasing. The petals and leaves of Camellia japonica L. have a certain reduction effect for irons, with the increasing of sample concentration, the reducing ability incresing to a dose-effect relationship, and the petals extracts reducing ability is 2-3 times than leaves at the same concentration.4. The results of active ingredients showed that polyphenol and flavonoid contents of n-butanol extract in petals were 27.92%,39.56%, respectively; The polyphenol and flavonoid contents of ethyl acetate extract were 32.32%,33.16%, separately; However polyphenol and flavonoid contents of n-butanol extract in leaves were 11.64%,21.53%, respectively; The polyphenol and flavonoid contents of ethyl acetate extract were 10.43%,13.16%, separately. The results indicated that the ethyl acetate and n-butanol extracts exhibited the strongest bile salt-binding capability and antioxidative effect, and the polyphenol and flavonoids content of ethyl acetate and n-butanol extracts were obviously much more abundant than the other three parts. The results revealed that there is a significant correlation between bile salt-binding capacity, antioxidative effect and polyphenol, flavonoids content.5. The results of active ingredients by LC-MS and HPLC analysis showed that n-butanol and ethyl acetate extracts contained catechin and epicatechin in petals and the contents of them up to 11.72% and 31.37% in n-butanol extract, separately; catechin and epicatechin contents up to 7.65% and 52.14% in ethyl acetate extract, respectively. Catechin and epicatechin have a strong antioxidant and lipid-lowering capacity and the lipid-lowering ability is 1-2 times than the same dose cholestyramine, antioxidant capacity is greater than Vc significantly.