| Objective: By establishing the model of rats with obstructive jaundice, we tried to observe the protective effect of SAMe(S-Adenosyl-L-methionene) on the liver function of rats with obstructive jaundice and to explore the possible mechanisms of SAMe on protecting liver cells. We hoped to provide a theoretical evidence for clinical biliary obstruction in patients with early liver protection drugs.Methods: By double wire suture at the lower end of the common bile duct, we established the model of obstructive jaundice rats successfully. Rats were randomly divided into for three groups: normal control group(n = 24), operation control group(n = 24), SAMe treatment group(n = 24). Rats in the SAMe group were given to SAMe according to 100 ml/kg/d abdominal cavity injection. The normal and operation control groups’ rats were injected with the same amount of normal saline. In the corresponding 3, 7, 10 and 14 days, we got liver tissues, and recorded the general situation of rats and liver general view. In each time points, we also detected in rat serum total bilirubin(TBIL), direct bilirubin(DBIL), alanine transaminase(ALT), aspartate aminotransferase(AST). The liver tissues pathology were detected by HE staining, and TUNEL assay was used to detect liver cell apoptosis. Immunohistochemistry and morphologic quantitative analysis were used to detect the expression of Bax, Bcl-2 and Caspase-3 protein.Results:(1) The general of liver tissue showed: In the normal control group, the liver tissue was normal, the color was red, and the edge was sharp. In the operation control group, the liver tissue of the rats and the edge of the liver were dull. It was brown and yellow, and the cystic dilation of the common bile duct gradually increased. In the SAMe treatment group, the liver tissue was slightly dark, and the extent of the lesion was mild.(2) Liver function showed: In the 10 and 14 days, ALT, AST, TBIL, DBIL levels of blood serum in the operation control group rats were significantly higher than the SAMe group content, the difference was statistically significant(P< 0.05).(3) HE staining showed: Normal liver tissue showed normal cell structure, clear liver bile duct distribution, and typical structure without bile duct proliferation. The rats of operation control group liver tissue under the microscope, the liver cells were spotty and patchynecrosis. Hepatic lobule structure showed varying degrees of damage due to the influence of bile duct hyperplasia. With the obstruction time prolonged, the changes of abnormal structure gradually increased. The liver structure of SAMe group was lightly damaged compared with the operation control group.(4) Immunohistochemistry showed: the protein of Bax, Bcl-2, Caspase3 mainly expressed in the cytoplasm of liver cells, and the nucleus had a small amount. In the experiment 7 or 10 days, compared with the control group, the expression of Bax and Caspase-3 protein in the liver cells showed a trend of decrease in the expression of in the SAMe treatment group. On the 10 days of the experiment, compared with the operation control group, the expression of Bcl-2 protein in the liver cells showed a rising trend in the SAMe treatment group.(5) TUNEL showed: In the experiment 10 and 14 days, compared with the operation control group, the apoptosis index of the liver cells showed a decreasing trend in the SAMe treatment group, P <0.05.Conclusion:(1) Exogenous SAMe can decrease hepatic enzymes and reduce bilirubin, which plays a protective role on the liver in rats with obstructive jaundice.(2) In the rat model of obstructive jaundice, SAMe can regulate the ratio of Bcl-2/Bax protein and down regulate the expression of Caspase-3 protein.(3) The protective effects of SAMe on the liver may be related to the process of hepatocyte apoptosis. |
PDF Full Text Request