Study On The Quality Control Of Atractylodes Chinensis From Chengde

Atractylodes is a traditional Chinese medicine in our country, which has a long history, beginning "shennong hundred rough market" of the Eastern Han Dynasty,and is listed as one of the top grade. Included in the "Chinese Pharmacopoeia", it resources from the dried roots of composite Atractylodes chinensis(DC.)Koidz or Atractylodes lancea(Thunb.) DC. [Indications]: drying damp and strengthening spleen, expelling wind chill,and improving eyesight.It can also use for damp retention in middle-jiao, abdominal distention,diarrhea,edema,atrophy furrowed beriberi, rheumatism, cold, night blindness,and eyes faint astringent.The wild resource of Atractylodes chinensis has beening endangered at p resent because of soaring requirement year by year. As the serious status that the resource of Atractylodes chinensis is very shortage, the scale and standardi zed implantation of Atractylodes chinensis is imperative.This topic was based on 2013 planting support project of Jingfukang pharmaceutical industry which obtained the approval of the ministry of “the north rhizoma atractylodis scale, standardization production demonstration base construction”. The paper takes chengde Atractylodes chinensis planting medicinal materials as the main research object, using gas chromatography and gas chromatography mass spectrometry technology to study wild and cultivation of Chengde Atractylodes chinensis chinensis and other market procurement Atractylodes chinensis samples. Then through a certain index content determination and feature maps, the paper improves the quality control level of Atractylodes chinensis, and provides a scientific basis for the North herb standardized, large-scale planting。Objective:1.Using gas chromatography-tandem quadrupole mass spectrometer(GC-MS) and Clustering analysis method, to analyse the similarities and differences of Atractylodes chinensis’ chemical constituents between Chengde and other origins(market purchase), for chengde cultivated Atractylodes chinensis providedthescientific base for further study ofchemical composition. 2.Establish the content determination method and characteristic spectrum by GC of atractylodin,atractylon,β-eudesmol of Atractylodes chinensis,so as to provide the basis for scientific evaluation of its quality. 3.To analysis the medicinal materials quality of Atractylodes chinensis from chengde cultivation of different years and different growth areas. 4.Accelerated storage tests have been used on rapidly study the shelf-life of Atractylodes chinensis,Analysis of atractylodin, atractylone, β- eucalyptus content under accelerated storage conditions, Preliminary Study on effects of storage time and storage conditions on the quality of Atractylodes chinensis.Methods: 1.Using GC-MS to analyse the samples mentioned above, qualitative and quantitative analysis of all tested chemical constituents were made, followed by a cluster analysis between different samples. 2.Using GC, Agilent HP-5 capillary column, nitrogen as carrier gas, FID as detector, temperature programmed, split ratio, injection port temperature: 250℃, detector temperature: 250℃, column temperature: 130℃;Using External standard method Determinates characteristic spectrum ofatractylodin, atractylon and β-eudesmol of 25 samples between chengde and other market purchase, Set characteristic spectrum and utilize the Estimating System of Similarity on the Chinese Medicine Fingerprint Chromatogram to make similarity analysis. 3.With the "Method 2" establishing chromatographic conditions and testing methods, I analyzed the mass of northern herbs which have different growth years and areas, planting from Chengde root or the seeds of medicine. 4.Using accelerating test with incubator under a humidity of 70% and temperature of 40℃, I studied the northern herb medicine storage stabilitywith the "Method 2" establishing chromatographic conditions and testing methods.Results: 1.A total of 35 chemical compounds were detected in all of the Atractylodes chinensis samples, and 33 of them were identified, which included 13 kinds of olefin, 9 kinds of alcohols, 6 kinds of ketone, 5 kinds of ester and 2 kinds of aromatic hydrocarbons. Cluster analysis results showed that Atractylodes chinensis from Chengde and Inner Mongolia belonged to one class.Atractylodes chinensis from Liaoning, Jilin and Shanxi belonged to the other. 2.The content determination method and characteristic spectrum was establish by GC of three main constituents of Atractylodes chinensis, identifying nine characteristic peaks. The linear range of atractylodin was 16.46 ~ 329.3 μg/m L(r = 0.999 6), and the average recovery was 100.12%, RSD = 0.88%(n = 6), and the limit of detection was 0.06 ng. The linear range of atractylon was 35.00 ~ 348.7 μg/m L(r = 0.999 5), and the average recovery was 99.84%, RSD = 1.29%(n = 6), and the limit of detection was 0.04 ng. The linear range of β-eudesmol was 20.00 ~ 406.1μg/m L(r = 0.999 9), and the average recovery was 100.75%, RSD = 1.17%(n = 6), and the limit of detection was 0.12 ng. 3.Root transplanting Atractylodes chinensis: examines the growth levels from 0.5 year to 2 years, including rhizoma atractylodis element content, Atractylodeschinensis ketone content.the content of rhizoma atractylodis element and rhizoma atractylodis ketone is flat(excluding individual), and close to wild samples’ growth;β‐eucalyptus alcohol content is downward trend( except individual). Atractylodes chinensis grown from seeds: examines content from 1 year to 3.5 years, including rhizoma atractylodis, Atractylodes chinensis ketone content is the trend of the growth, grow up to 3.5 years samilier to wild samples;β‐eucalyptus alcohol content overall content is big different from wild samples. 4. Atractylodes chinensis, β‐eucalyptus alcohol ketone content in storageperiod 0 ~ 6 months is on the decline;Rhizoma atractylodis element changes small.Conclusion: 1. Atractylodes chinensis purchased from Chengde and other market are differences in the amount chemical composition and the relative content components, but on the whole chengde north rhizoma atractylodis show no significant difference in the amount of chemical composition(32 to 35) and the relative content components. The amount chemical composition and the relative content components of different origin Atractylodes chinensis are significant differences. The cluster analysis showed that the amount chemical composition and the relative content components independent of the year of sample, and Chengde and Inner Mongolia north rhizoma atractylodis are similar in composition chemical composition. 2.The concent determination and characteristic spectrum method of atractylodin, atractylon and β-eudesmol established by this study is sensitive, simple, stability and make the determination result accurate and reliable. 3.Content of Atractylodeschinensis element, rhizoma atractylodis ketone, and the β‐eucalyptus alcohol were analyzed and the sample experimental data was compared with wild sample, reflecting that cultivation of north rhizome a tractylodis was feasible in a certain extent(The sample size is less, which nee ds investigation further). 4.Accelerated test shows that the quality of rhizoma atractylodis can be i nfluenced by the conditions and duration of the storage. This test laid a found ation for the future research of rhizoma atractylodis storage conditions.