| Objective: To observe the antioxidant effect of L-carnitine on tripterygium glycosides induced oligoasthenozoospermia rats and to discuss corresponding possible mechanism.Methods: 50 male SD rats were randomly assigned to 5 groups: the normal group,the model group, the L-carnitine group(low-dose), the L-carnitine group(mid-dose),L-carnitine group(high-dose), and each group with 10 rats. After one week adaptive feed, make models by the means of gavage: in normal group, distilled water were given every day; in the other groups: Tripterygium Wilfordii Polyglycoside(TWP)were given with the dose of 30mg/kg every day for 6 months for the modeling of oligoasthenozoospermia. After finishing the modeling, distilled water were given continuously within the normal group and the model group; About L-carnitine groups,different doses of L-carnitine were given among three groups, that were 100mg/kg in low-dose group, 200mg/kg in mid-dose group and 400mg/kg in high-dose group respectively, the used L-carnitine was made up with distilled water and continuously used for 4 weeks, the frequency was one time a week, the weight of rats in each group were recorded continuously too. 24 hours after the last-time drug delivery, put the rats to death by breaking the neck, take one side of the epididymis, strip needless adipose tissue and make them weighed to calculate epididymis index, drift away the sperm in the tail of epididymis to determine the sperm density,motility rate, activity and abnormality rate.take another side of the epididymis, make it into homogenate and finish MDA,SOD and CAT text.Results:1. The sperm density, motility rate and activity in the model group were obviously lower than that in the control group(P<0.05) while higher in L-carnitine group than that in the model group(P<0.05), besides, when compared L-carnitine group with control group, there was no statistical significance(P>0.05) in mid-dose group andhigh-dose group and were statistical significance(P<0.05) between low-dose group and control group, mid-dose group and high-dose group.2.The MDA concentration of the rats’ epididymis homogenate in the model group is significant higher than that in the control group and L-carnitine group(P<0.05); there was no statistical significance(P>0.05) when compared MDA concentration in mid-dose group and high-dose group with that in control group; while the content in low-dose L-carnitine group was higher than that in the control group and other two L-carnitine group(P<0.05).3. The SOD and CAT concentration of the rats’ epididymis homogenate in the model group is significant lower than that in the control group and L-carnitine group(P<0.05); there was no statistical significance(P>0.05) when compared SOD and CAT concentration in mid-dose group and high-dose group with that in control group; while the content in low-dose L-carnitine group was lower than that in the control group and other two L-carnitine group(P<0.05).Conclusions: L-carnitine can improve the activity of antioxidase, enhance resistance and repair ability of spermatogenic cells towards oxidative stress injury, protect the sperm from the over attack of reactive oxygen species and finally improve the quality of sperm. |
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