Compsition Of Flavonoids In Physalis Peruviana L. And The In Vitro Activities

The study was performed for Physalis peruviana L. Flavonoids, it includes flavonoids component analysis and physiological activity in vitro. Physalis peruviana L. treated as raw materials fruit juice, fruit powder, extraction of flavonoids from it and extracted by petroleum ether, trichloromethane, ethyl acetate, butyl alcohol.Using LC-MS to detect detection material components of each extract phase. It detected each extract phase containing Genistein, Luteoloside. The trichloromethane phase also contains Luteolin, Naringin, Ombuin, Poncirin, Scutellarin. In contrast, the ethyl acetate phase containing Luteolin, Marigold prime-glucuronide.Physalis peruviana L. and its extract were done in vitro antioxidant activity. It uses the method of DPPH, ABTS, T-AOC and ORAC. Physalis peruviana L. extract trichloromethane phase has a strong scavenging capacity, higher total antioxidant capacity and ORAC value. When the concentration of 3 mg/mL of ABTS radical scavenging capacity of 0.4194±0.015 mmol/g, total antioxidant capacity and ORAC values were 12.06±0.57 mg/mL,9.656±0.34 mmol TE/g, wherein ABTS radical scavenging ability is stronger than the positive control Vc (0.1147±0.010 mmol/g). When the concentration of 4 mg/mL, ethyl acetate phase DPPH·scavenging capacity of 90.67%, slightly lower than the VC (93.03%), but higher than other samples.Research on each sample in vitro inhibition of enzymes, fruit juice, fruit powder, petroleum ether phase, trichloromethane emulsion layer phase, and the aqueous phase is very weak compared to other samples of enzyme inhibition ability. The ethyl acetate phase has a strong inhibitory effect on a-glucosidase, a-amylase. At a concentration of 14 mg/mL, the inhibition rate was 68.37%,68.70%. The trichloromethane phase has a good inhibitory effect on the lipase. At the same concentration, the inhibition rate was 71.08%, slightly higher than the concentration of 11 mg/mL of acarbose (69.09%).