| Objective:KuBai Granule observed croton oil induced inflammatory effects hemorrhoid surgery and IL-2, IL-6, the impact of the proinflammatory cytokines IL-8’sMethods:Croton oil-induced inflammatory agents hemorrhoid postoperative inflammation rat model of Parkinson particles treated group were KuBai Granule, divided into high, medium and low dose group and control group to select 1: 5000 potassium permanganate, rinse 20 min daily, continuous seven days, the model control group with saline. On the fourth day, the seventh day do take the anal tissue biopsy incision wound, granulation observed in each group of rats, the intensity of inflammatory cells; canthus blood 2.0ml seventh day after surgery rats in each group head, take serum, according to kit instructions, measurement of serum IL-2, IL-6, IL-8 content by ELISA.Result:(1) bitter cedar particles lotion in promoting the growth of granulation, inhibition of wound tissue edema, exudation side effect is significant, the effect was positively correlated with the concentration.(2)Pathological examination showed that: rat fourth day after modeling, high KuBai Granule, the intensity of the inflammatory cell dose group was significantly loose, significantly reducing the number, while the low dose group suffering Parkinson particles dense inflammatory cells, reduce the number of obvious. The seventh day after the model rats, KuBai Granule sparse inflammatory cells, high-dose group, high-power microscope only a few inflammatory cells per field, cypress bitter dose group sparse inflammatory cells, bitter cedar low dose group, high manganese potassium group show pathological inflammatory cells decreased, indicating that bitter lotion Parker particles compared with potassium permanganate can inhibit inflammation, anti-inflammatory effect was positively correlated with the concentration.(3) Parkinson bitter lotion particles high dose group of wound tissue in inflammatory mediators IL-2, IL-6, IL-8 and potassium permanganate group, model control group was highly statistically significant(p <0.01); KuBai Granule low dose group of wound tissue inflammatory mediators IL-2, IL-6, IL-8, compared with the control group was highly statistically significant(p <0.01), groups were compared with potassium permanganate there was statistically significant(p <0.05).Conclusion:the Ku Bai lotion to particles by inhibiting inflammatory mediators IL-2, IL-6, IL-8 release was significantly inhibited croton oil induced inflammatory response in rats after anal fistula surgery, anti-inflammatory effect. |
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