The Effect Of Biocompatibility Of Pure Magnesium With Synergy Treatment Of Micro-arc Oxide,NaOH Alkali And Silane-silk Fibroin Modification

Objective: In view of the weak corrosion resistant and low activity of the micro-arc oxide coating for the pure magnesium materials,this paper developed the new technology of microarc oxide method,NaOH alkali treatment and surface modification with silane-silk fibroin films.The effect of the different treatment on the osteoblast adhesion and proliferation behaviors were investigated in details.This new method will provide clinical basis reference for the new type of medical implant materials.Processing methods: The material with ultrasonic micro-arc oxidation treatment was defined as contrast group sample(Group A),and the experimental groups(Group B)and(Group C)were immersion treatment 30 min and 90 min respectively by silk fibroin solution after the combined treatment with micro-arc oxide method,NaOH alkali treatment and surface modification with silanization.The amentioned three groups material were experimental characterization by growth,adhesion and proliferation of osteoblasts.The microscope film surface morphology and cell early formation were characterized by means of scanning electron microscope and laser scanning con-focal microscope.The osteoblast proliferation and activity condition was detected by the CCK 8 and ALP methods.Results: The growth and adhesion condition of osteoblasts for the experimental groups was better than that of contrast group.In the meantime,Group C had the best condition in the three groups.The aforementioned compared results was satisfied with statistical methods.Conclusion: The biocompatibility has improved by surface modified treatment of MAO coating combined with NaOH-silane-silk fibroin combined treatment.The biocompatibility of the pure magnesium material with NaOH-silane-silk fibroin combined improved,especially for the silk fibroin dipping treatment for 90 min.