Research Of Biocompatibility Of Pure Magnesium Ultrasonic Micro-arc Oxidation-NAOH(HF)- Silane Coupling Compounded Treatment.

Objective:Magnesium is one of the minerals necessary to sustain the body’s normal, is indispensable to human body elements, the research shows that if the lack of magnesium in the body, will cause many diseases, so it can be seen that magnesium metal material on the importance of the human body. And micro-arc oxidation technology, is the most recent was looking forward to the surface of the coating technology for the last few years, is made by electric pulse and heavy metal solution to match. The advantages of micro-arc oxidation has a lot of, can form biofilms, a kind of ceramic layer, can better make the integration of material, combining the higher, increase the wear resistance and hardness of the material, biological membrane has a good insulation, and low production cost. Due to the degradation of pure magnesium too fast, micro-arc oxidation technology can make a layer of ceramic coating on the magnesium surface to reduce the degradation velocity of magnesium, improve the biocompatibility of magnesium.But the micro-arc oxidation surface of pure magnesium tiny pore formation, but also not conducive to the practical stability of materials. So aiming at the existence of pure magnesium micro-arc oxidation coating porosity, research a kind of can improve existing in the micro-arc oxidation coating of the pore, more improve material biocompatibility. After material research found that the silane treatment can obviously improve the porosity of the material form, this treatment is a new kind of technology, it can greatly improve the adhesion on the surface of the metal and the pure magnesium by micro-arc oxidation- hydrofluoric acid- silane and micro-arc oxidation- sodium hydroxide- silane coupling post-processing cell compatibility of materials.Methods: The experiment was divided into three groups, the pure magnesium micro-arc oxidation is used as the group A, the pure magnesium micro-arc oxidation-HF- Silane coupling is group B, the pure magnesium micro-arc oxidation-NAOH- Silane coupling is group C.Adopt CCK-8 to test the osteoblast value-added, testing cell activity in alkaline phosphatase, confocal laser detecting osteoblast adhesion ability, scanning electron microscope watching cell adhesion.Results through the CCK8 experiment cell proliferation, can be seen by scanning electron microscope to observe cells, extend through SEM observation of cell adhesion, three sets of samples with the increase of time, cell adhesion, growth and differentiation ability are improved; But at the same time, the outcome of the group C is significantly higher than group B, group B results higher than that of group A.Conclusion: The cell compatibility of pure magnesium micro-arc oxidation after disposal is better than pure magnesium micro-arc oxidation material,the biological compatibility of pure magnesium micro-arc oxidation- Na OH- silane coupling composite treatment is best.