| The security of drinking water is always a vital matter for the people and also the country. Recent years, the pollution of water becomes more and more serious along with frequent terrorist events, for the sake of safety and benefits of the people, it’s necessary to establish a quick and efficient on-line detection system for drinking water which becomes one of the significant research topics around the world.Colchicine was studied as a potential contaminant in this thesis. It’s the way that luminol- potassium ferricyanide chemiluminescence system combined with flow injection technique to build up the on-line detection and warning system for the pollution of colchicines in drinking water. The details were as follows:(1) The physical and chemical properties after the pollution of colchicines in drinking water;(2) The cytotoxicity of colchicines in vitro experiment;(3) The optimization of detection effect for luminol- potassium ferricyanide system by the response surface method;(4) The optimization of extraction of colchicines in day lily and lily;(5)Decomposition and removing methods of colchicine in drinking water.Main results were as follows:1. In the range from 0.1 to 1000 mg/L, as the increase of the concentration of colchicines, the chemical oxygen demand(COD) of drinking water was highly increased, while the conductivity, turbidity and p H values were increased slightly.Colchicine had little effect on common index of drinking water, so it’s very difficult to detect the pollution of colchicines, indicating high concealment which needed a further preventive method.2. Colchicine showed a time and dose dependent in vitro cytotoxicity on He La, HEK,A172 cells which was considerable strong, the results of IC50 for 24 h MTT experiment were respectively 3.23 ppm, 4.02 ppm, 6.75 ppm; while the Neutral red experiment were 2.12 ppm, 2.85 ppm and 2.57 ppm.3. The fluorescence of luminol- potassium ferricyanide system could be strongly suppressed by colchicine, this theory could be used to establish a flow-injection chemiluminescence detection method of colchicines. Detection conditions were optimized by response surface method, the best test condition were as follows: 1m L/min for pump speed, p H 13.20 for luminol solution, 3.00 × 10-4 mol/L for luminol solution concentration, 3.00 × 10-4 mol/L for potassium ferricyanide solution concentration. The linear range of the method was 5.0×10-3 ~ 1.0 mg/L,detection limit was 3.01×10-5 mg/L and 2.78% for RSD(n = 11).4. Organic solvent assisted with ultrasonic had been used to extract colchicines in day lily. It was extracted 12.4 mg colchicine in 50 g day lily powder and the content was 0.025%. Solvent concentration, solid-liquid ratio, temperature were selected to do response surface optimization test, the optimal extraction conditions were: 100% ethanol concentration, solid-liquid ratio 1:25, extraction temperature45℃, the extraction would be 17.3 mg. This result verified reliable.5. Under the indoor light, there was a certain degree of decomposition of colchicine,after 7 days remaining rate was 65%, while under strong sunlight, the decomposition rate was greatly increased, only 2 days later the remaining was11%; Ultrasonic method and the rod activated carbon adsorption treatment had limited effect in removing the colchicine. However, using powdered activated carbon at the addition 0.5 g/L with 5 min showed great removing effect, the remaining rate was 3%. |
PDF Full Text Request