Experimental Study Of Zhuang Medicine Zi Le Tiao Jing Decoction In Rats With Diminished Ovarian Reserve

Objective: By establishing the rat model with poor ovarian reserve function,to make up for Progynova,kuntai capsule for comparison,observation of ovarian morphology,sex hormone of rats and OSM,M-CSF cytokines and other related indicators,preliminarily Zi Le Tiao Jing Decoction to improve even restore ovarian reserve function mechanism.Methods: Sexual maturity,estrus normal 70 female SD rats,with random method is divided into modules?60?and blank control group?10?.Build module in tripterygium wilfordii more glucoside piece according to the 50 mg/?kg·d?dose,15 ml?kg·d?volume 2 weeks lavage mode,since lavage 6 days,every line of vaginal exfoliated cells smear observation records,evaluate the rat model.After the success of the building,with random method to build module is divided into TWP model group,kuntai capsule,Progynova group,Zi Le Tiao Jing Decoction of low dose group,Zi Le Tiao Jing Decoction of medium dose group,Zi Le Tiao Jing Decoction of high dose group,to fill the stomach medication intervention for 15 days,parallel vaginal exfoliated cells smear observation records.The anesthesia in rats after treatment,the abdominal aorta in blood serum separation,ovaries and uterus weighing and formaldehyde fixed immediately.Observation rat survival and general condition,body weight,estrus,gonad index,ovarian pathological organization form,it with free to measure each rat serum FSH,LH,E₂ and P levels,using immunohistochemical method to detect OSM,M-CSF level expression in ovarian tissue.Results:1.TWP model group estrogen cycles is far lower than the blank control group,the difference is statistically significant?P<0.001?,the treatment group estrogen cycles than the TWP model group were different degree increase,the difference is statistically significant?P<0.05?,including AIDS,regulate the menstrual function decoction high dose group of estrogen cycles closest to the blank control group.2.Compared with blank control group,TWP rat ovaries,uterus index of model group were lower,the difference is statistically significant?P<0.05,P<0.001?.Each treatment group with TWP than the model group,the ovary,uterus index were different degree increase,the differences were statistically significant?P<0.05,P<0.001?.Zi Le Tiao Jing Decoction ovarian index under low dose group rats kuntai capsule group,with statistical significance?P<0.05?,Zi Le Tiao Jing Decoction in high dose group of rats uterus index higher than that of kuntai capsule group,difference has statistical significance?P<0.05?.Zi Le Tiao Jing Decoction in low dose group than fill jiale rat uterus index is low,statistically significant difference?P<0.05?.Zi Le Tiao Jing Decoction in high dose group of the ovary,uterus index is higher than the low dose group,the uterus index above middle dose group,the differences were statistically significant?P<0.05?.3.Compared with blank control group,TWP growth follicle,mature follicle and corpus luteum model group were less,atresia follicles,the differences were statistically significant?P<0.001?,Zi Le Tiao Jing Decoction in low dose group of follicle growth,mature follicle and corpus luteum number are less than the blank control group,and the differences were statistically significant?P<0.05?.Kuntai capsule group,jiale,Zi Le Tiao Jing Decoction compared with TWP model group,high dose group,growth follicle,mature follicle and corpus luteum number has increased to reduce atresia follicles in different degree,the difference had statistical significance?P<0.05,P<0.001?,Zi Le Tiao Jing Decoction growth follicle than TWP model group,low dose group differences statistically significant?P<0.05?,the number of mature follicle and corpus luteum and atresia follicles are improved,but no statistically significant difference?P>0.05?.Zi Le Tiao Jing Decoction high dose group in the mature follicle and corpus luteum number more than the low dose group,the difference statistically significant?P<0.05?.4.The treatment group compared with TWP model group,microscopical-ly,the ovarian structure is clear,all levels of follicle and corpus luteum number,granular cell layers,and the blank control group,atresia follicles and white body.5.TWP model group rats serum FSH,LH level is high,low level of E₂,compared with the blank control group statistically significant difference?P<0.001?,Zi Le Tiao Jing Decoction of low dose group after treatment,Zi Le Tiao Jing Decoction of rats FSH levels fell,E₂ levels increased,but compared with the blank control group,difference has statistical significance?P<0.05?.Each treatment group rats after treatment,the serum FSH,LH level was also decreased to different degrees,E₂ levels increased to varying degrees,compared with TWP model group,including kuntai capsule group,jiale,zi le regulate the menstrual function in the soup,the high dose group were statistically significant difference?P<0.05,P<0.001?.Zi Le Tiao Jing Decoction of high dose group of serum E₂ levels higher than the low dose group,difference has statistical significance?P<0.05?.6.Zi Le Tiao Jing Decoction of low dose group,TWP model ovarian OSM IOD values than with the blank control group,difference has statistical significance?P<0.001?,the treatment group of ovarian OSM IOD values were significantly increased,compared with TWP model group,the differences were statistically significant?P<0.001?.Zi Le Tiao Jing Decoction in low dose group OSM IOD values below kuntai capsule group and fill jiale group,the differences were statistically significant?P<0.05?.Zi Le Tiao Jing Decoction of high dose group of OSM IOD values higher than the low dose group,the difference is statistically significant?P<0.05?.7.Zi Le Tiao Jing Decoction in low dose group,TWP model M-CSF IOD values and ovary than blank control group,the difference is statistically significant?P<0.001?,ovarian M-CSF IOD values in each treatment group were significantly increased,compared with TWP model group,the differences were statistically significant?P<0.001?.Zi Le Tiao Jing Decoction in low dose group M-CSF IOD values below kuntai capsule group and zi le regulate the menstrual function decoction high dose group?P<0.05?.Conclusion: Using tripterygium wilfordii glycoside made are more rats model and use of kuntai capsule,Progynova,Zi Le Tiao Jing Decoction of three dose treatment had no obvious effect on body weight of rats.Zi Le Tiao Jing Decoction can increase are the viscera index of the ovaries,uterus,increase the number of follicle and corpus luteum at all levels,reduce atresia follicles,can through the lower levels of serum FSH,LH,E₂ level rising to regulate hormones,increase the OSM,M-CSF expression in ovarian tissue,promote follicular growth,this could be one of the mechanism of prevention and cure are.Kuntai capsule,Progynova,and Zi Le Tiao Jing Decoction can improve the ovarian reserve function,promote follicle growth and maturity.Zi Le Tiao Jing Decoction is dose dependent,the dose and effects of kuntai capsule,Progynova most,is the most appropriate doses in three doses.