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Studies On The Water Soluble Constituents Of Poten Tilla Anserina L. And Lycium Barbarum L.

Posted on:2018-07-29Degree:MasterType:Thesis
Country:ChinaCandidate:P F WuFull Text:PDF
GTID:2334330536980544Subject:Pharmaceutical engineering
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Potentilla anserina L. belongs to Rosacrae Potentilla is the root tuber of Potentilla Potentilla. It is a common Tibetan Medicine and al so a kind of Health care product. This paper explores the Potentilla Potentilla water-soluble part in order to improve the study on chemi cal constituents of Potentilla anserine plants.In this paper, MCI colu mn chrom-atography,HW-40 column chromatography,ODS column c hromatography and preparative HPLC chromatography separation tec hnology was used again and again.The water part of 60% acetone ex tract and n-butanol part were ,tudied, 15compounds were isolated. 15 compounds were identified by modern spectroscopic techniques, all o f them were known compounds .They were identified as Epigallocatec hin-7-O-β-D-glucoside(1),cate chin-7-0-β-D-glucoside(2),epic at echin-7-O-β-D-glucoside(3),six hydroxy-3- retinol(4),epigallocatechin(5),proanthocyanidin B3 (6),epig alloc at echin(4-alpha,8)-epigallocatechin(7), ( + )-catechin(8),2R,3R-3,5,6,7,3’,4’-hexahydroxyflavane(9),( + )-e picatechin(10),hy-droxytyrosol-4-O-β-D-glucoside(11),Isotachioside(12),proanthocyanidinC(13),kajiichigoside F1(14),pomolic acid-28-O-β-D-glucoside(15). Compoundl-13 was isolated from this plant for t he first time.Lycium Barbarum L. belongs to Solanaceae. Lycium barbarum is used as a traditional Chinese medicine and functional food in China.In this paper, a systematic study was carried out on 60% acetone extr acts of Lycium barbarum L.The repeated use of MCI column chromat ography, HW-40 column chromatography,ODS column chromatograph y and preparative HPLC chromatography,60% acetone extract of Lyci um barbarumwere studied,6 compounds were isolated,The structures of 6 compounds were identified by modern spectroscopic techniques and literature review.They were identified as 5-(hydroxymethyl)-1-(2-hydroxypropanoyl) -1H-pyrrole-2-carbaldehyde(1),phaseic acid(2),tr ans-p-coumaric acid 4-O-β-D-glu-copyranoside(3),Benzenepropanoic acid,4-(β-D-glucopyranosyloxy)-3- methoxy(4),2-Propenoic acid,3-[4-(β-D-glucopyranosyloxy)-3-methoxyphenyl](5), 1 H-Pyrrole - 1-butano ic acid,2-formyl-5-(hydroxymethyl)(6). Compound2-5 was isolated fr om this plant for the first time.Compound(l)is new.
Keywords/Search Tags:Potentilla anserina L., Lycium Barbarum L., water solu ble constituents, catechin, 5-(hydroxymethyl)-1-(2-hydroxypropanoy 1)-1H-pyrrole-2-carbaldehyde
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