| BackgroundBlumea balsamifera is compositae einar genus Blumea balsamifera(L.)DC.Plants perennial herbs or sub shrubs,has a long history of the medicinal in the folk,has a unique efficacy in treating dysfunctional uterine bleeding and postpartum hemorrhage etc card,it proved that the einar sweet element such as flavonoids have better the bleeding and clotting activity,but its mechanism remains to be further explore,Blumea balsamifera as many of the newly found terpene compounds,amino acids and polysaccharides are showed certain biological activity,but its extraction technology and pharmacological activity of research is still at the initial stage,has good research prospect,at the same time,further revealing the main active ingredients in einar fragrance helps us to determine the quality standard of medicinal materials,rational utilization and development of einar resources,so as to create more value.Objective(1)To establish a fingerprint map of Blumea balsamifera and to provide reference for its quality control;(2)To explore the anti-oxidative activity and anti-inflammatory effect of the different polar active sites of Blumea balsamifera,and to lay a theoretical foundation for the study of pharmacodynamics in the later stage.Methods(1)By investigating the chromatographic conditions of the flow phase system and the treatment method,the method of precision test,stability test and repeatability test were studied.(2)Based on the fingerprint atlas of ainaxiang,the system cluster analysis,principal component analysis and similarity analysis were carried out by the method of stoichiometric analysis.(3)Different polarity active site were maked,by preparation of einar using Prussian blue method,o-phenanthroline methods,the DPPH method respectively,einar different polarity active site of reducing power,hydroxyl radical scavenging,DPPH radical removal ability,and comprehensive evaluation of einar incense in vitro antioxidant activity were investigated.(4)RAW264.7 cells were randomly divided into 8 groups: blank group,model group(LPS induced)group and petroleum ether group,chloroform group,ethyl acetate parts group,n-butyl alcohol group,water area group,the crude extract group,which were respectively given media,LPS,LPS + petroleum ether part,LPS + chloroform parts and LPS + ethyl acetate parts,LPS + n-butanol part,LPS + water area,LPS + crude extract,together with the cell incubation for 24 h.the level of NO,TNF-alpha and IL-6 in the cell supernatant were determined.Results(1)The chromatographic condition of the fingerprint of Blumea balsamifera was determined,and Methodological investigation test showed that RSD were all less than 3.(2)There were two main categories for the 13 samples,the results of principal component analysis showed that according to Extracting principle with eigenvalues greater than 1 component,the two principal components were extracted.(3)With the increase of concentration of sample,clearance ability of DPPH free radicals,reducing power,clear rights capacity of hydroxyl radicals in the different active site of Blumea balsamifera increased,which existed a good linear relationship with the sample concentration.The reduction ability of ethyl acetate layer sample,n-butyl alcohol layer,petroleum ether layer,trichloromethane layer and water layer is significantly less than the reduction ability of ascorbic acid Vc.The removal of hydroxyl radical in ethyl acetate layer was the strongest,and the second was n-butyl alcohol layer.(4)Compared with the blank group,the level of NO,tnf-alpha and IL-6 in the cells of the model group was significantly higher than that in the blank group(P<0.05).Compared with the model group,Compared with the model group,the different polar regions of Blumea balsamifera can reduce the level of inflammation factor NO,TNF-alpha and IL-6 in different degree.Conclusion(1)The mobile phase system,the processing methods such as chromatography condition were greatly determined,precision test,stability test,repeatability test methodology investigation results showed that the method had high accuracy,good stability.It was suitable for the study of the fingerprint atlas of Blumea balsamifera.(2)It may not be the same quality in different habitats,but the quality may be different in the same province.(3)The different active sites of Blumea balsamifera had certain anti-oxidative activity in vitro,and different active sites had different antioxidant activity abilities in vitro,among them,ethyl acetate extract and n-butanol extract have strong antioxidant activity effect in vitro.(4)The different active sites of Blumea balsamifera had certain anti-inflammatory effects,and different active sites had different anti-inflammation abilities in vitro,Among them,ethyl acetate extract has strong anti-inflammatory effect in vitro. |
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