Semi-targeted Analysis Of Unknown Migrating Materials In Food Contact Materials

In recent years,the issue of food safety has attracted more and more attention.However,unknown compounds leached from food packaging materials are almost unknown or ignored by consumers.The migrants of food packaging materials are characterized by great variety,low concentration,and rapidly increased amounts.Traditional target analysis method is not competent for the increasing number of unknown migrants.Phenols,amines,aldehydes and acids migrated from food packaging materials can rarely be determined by liquid chromatography because they show neither ultraviolet absorption nor fluorescence property.In this study,a semi-targeted new method was developed for the analysis of unknown migrants from food packaging materials on the basis of selective fluorescence labeling and mass identification.The introduction of specific fluorophore into the target molecules made the high performance liquid chromatography(HPLC)analysis of phenols,amines,aldehydes and acids migrated from food packaging materials become possible,meanwhile the HPLC sensitivity was also greatly enhanced.Online mass spectrometer was used for the identification of the unknown compounds.The contents were as follows:In chapter one,the general status of food safety and green analytical chemistry was mainly described.Some green extraction methods in sample pretreatment of analytical chemistry were presented.The development and application of high performance liquid chromatography and fluorescence enhancement was summarized.In chapter two,A self-made green and efficient semi-targeted method which including green gas purge microsyringe extraction(GP-MSE),pre-column derivatization HPLC with fluorescence detection and mass confirmation was developed for the analysis of aliphatic amines in food packaging materials.It improves the extraction efficiency of food packaging materials,which can amplify the signal intensity effectively.At the same time,the use of 10-ethyl-acridone-2-sulfonyl chloride(EASC)with specific fluorescent property greatly improved the accuracy and sensitivity of analysis.The developed method was characterized by celerity,accuracy and high sensitivity.This method makes it possible for the compatibility of sample and water.It was successfully applied to the determination of aliphatic amines in varied food packaging materials.In chapter three,a new method was developed for the analysis and confirmation of aliphatic aldehydes from different kinds of food packaging materials by using a self-made gas purge microsyringe extraction(GP-MSE)system in combination with HPLC-MS.The 2-(5-Benzoacridine)ethyl-p-toluenesulfonate(BAETS)with excellent fluorescence property was applied to enhance the HPLC sensitivity.The results indicated that formaldehyde and acetaldehyde were detected in high frequency from food contact materials.In chapter four,a new method was developed for the analysis and confirmation of volatile carboxylic acids(VFAs)and perfluorocarboxylic acids(PFCAs)in food packaging materials by using gas purge microsyringe extraction(GP-MSE)system in combination with HPLC-MS,which 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide(BAAH)was used as the fluorescent reagent.In this method,a dispersive liquid-liquid microextraction(DLLME)was used to the enrichment and purification of extraction solution.In chapter five,a method of solid phase extraction was developed to analysis the phenolic compounds in food packaging materials.In this method,a new home-made aminopropyl-functionalized SBA-15(N-SBA-15)mesoporous material was used for the extraction of the analyte.HPLC with fluorescence detection was applied for the analysis of the extracted analytes.The results indicated that bisphenol A and 2,6-di-tert-butyl-4-methylphenol were detected in high frequency from food contact materials.