Study On Antioxidant Activity And Mechanism Of Xuefu Zhuyu Oral Liquid

Xuefu Zhuyu Oral Liquid(XZOL)is derived from Xuefu Zhuyu Decoction,one of the top ten ancient recipes in China.According to previous research on it,its main effect is to improve the symptoms of insomnia,multiple dreams,internal congestion,irritability and anger,etc.The investigation found that some patients with myocardial ischemia,hypoxia and coronary heart disease have relieved their symptoms when taking Xuefu Zhuyu Oral Liquid.Detection,to explore its role in adjuvant treatment of coronary heart disease.In this laboratory,Xuefu Zhuyu Oral Liquid was used as the experimental material to test its anti-oxidation effect in vitro by detecting its ability to scavenge free radicals in vitro;mouse mononuclear macrophages(RAW264.7)were used to simulate cell inflammation,To detect the effect of Xuefu Zhuyu Oral Liquid on cell survival rate and cell ROS content,to explore its cellular level of anti-oxidation;using nematodes as a model organism,to detect the life span,health status and stress ability of Xuefu Zhuyu Oral Liquid And other aspects,to explore its anti-oxidant effect in vivo.1.Study on scavenging free radicals outside Xuefu Zhuyu oral liquid.By measuring the scavenging ability of different concentrations of XZOL on ABTS free radicals,DPPH free radicals,hydroxyl free radicals and superoxide anions,the ability of scavenging free radicals in vitro was judged,indicating that Xuefu Zhuyu Oral Liquid has good antioxidant properties.2.Study on cell-level antioxidant of Xuefu Zhuyu oral liquid.Cellular inflammation model was established by lipopolysaccharide(LPS)-induced mouse monocyte macrophages(RAW264.7).The effect of different concentrations of blood XZOL on the survival rate of RAW264.7 cells induced by LPS and the effect of LPS on The effect of ROS content in cells indicates that Xuefu Zhuyu Oral Liquid has good antioxidant properties at the cellular level.3.Antioxidation of Xuefu Zhuyu oral liquid.Using nematodes as model organisms,the effects of different concentrations of Xuefu Zhuyu Oral Liquid on nematode lifespan,body fat level,lipofuscin level,motor function,oxidative stress and heat stress conditions were discussed.The effect of the metabolic process of E.coli on the biological activity of Xuefu Zhuyu Oral Liquid indicates that Xuefu Zhuyu Oral Liquid has good antioxidant properties in the body.