The Study Of The Origin And Development Of Adolescent Emotional Abnormal Pathogenesis And Its Experimental Treatment From Liver

BackgroundIn this paper,the system has been used to sort out the origin and main literature of "children’s emotional abnormalities" and "liver often more than"theory,combined with the characteristics of physiological and pathological changes of children and growth factors,and in this group has been fully confirmed can be effective on the basis of psychological stress depression,clear shock,fear,anger and other negative emotions is to promote the main cause of emotional disorders in children.Combined with pediatric adolescents"liver often more than" the pathophysiological characteristics,summed up youth emotional abnormalities must be treated from the liver.The main mechanism of the antidepressant diagnosis and treatment of traditional Chinese medicine was revealed by the traditional Chinese medicine Jiawei Sini Powder,and the mechanism of depression in adolescents was clarified,which could provide reference for further seeking effective intervention and clinical research.ObjectiveThis study summarizes the development of pediatric "liver often" theory,and takes the chronic unpredictable stress animal model(CUMS)of children with young children to simulate the state of external stimuli in children.By using traditional Chinese medicine Jiawei Sini Powder,The effect of prescription and the way of prevention and treatment of traditional Chinese medicine,and provide the experimental basis for the clinical application of Jiawei Sini Powder in the treatment of young and middle-aged emotional diseases,and further elucidate the abnormal relationship between young and young children Liver treatment,the prevention and treatment of Chinese and English teenagers emotional abnormalities of the development of great significance.MethodsRats were randomly divided into model group,blank control group,fluoxetine intervention group and Jiawei Sini powder group.Take the chronic unpredictable stress model,shackles,electric shock,light and strobe lights,fasting 24h,crowded,noise,cage cage tilt,24 hours of water,wet environment,odor stimulation,day and night upside down,acoustic stimulation 12 kinds of ways,13 kinds of modeling methods,two daily random,general morning and afternoon arrangements for a variety of stress methods occurred about 2～4 times,so that animals can not predict the occurrence of stress stimuli,the same should The method can not be repeated.21d began to model to 43d,the first 44d drawn;44d-55d normal feeding is not modeling and administration,to the first 56d drawn;the first 57d,continue to take a chronic unpredictable stress model modeling 21d,at 86d.The distribution,morphology and quantity of 5-HT1AR and 5-HTT positive cells in hippocampus were observed by immunohistochemically staining.The expressions of 5-HT1AR and 5-HTT positive cells were observed by RT-PCR.To observe the changes of 5-HT1AR expression in rats with chronic unpredictable stress.Results1.General condition:After 21 days of chronic unpredictable stress modeling,in addition to the blank group,the other groups of rats.The degree of activity was significantly lower than the blank group,the hair lacks luster,slow response to the sound,breeder close to the fear of Baotuan together after the performance of the mouth;jointing and stress modeling serious,irritability,stool loose forming;fluoxetine Group and Jiawei Sini powder group were active,rat hair clean and tidy and shiny,sensitive to the sound reaction,stool formation;intragastric and stress modeling fluoxetine group appeared more severe resistance and fear,the Chinese medicine group no The performance.2.Weight:Before modeling,there was no difference in body weight between rats in each group.Before modeling,rats in each group.There is no difference in weight.After the model was started,the rats in the adolescents had different degrees of weight.Compared with the blank group,the body weight of the other three groups of chronic stress rats decreased in different degrees.The results of the model 21d suggested that(P<0.01).Compared wi th the blank group,the body weight of the rats in the model group was significantly higher than that in the control group(P<0.01),and the body weight of the rats in the model group and the traditional Chinese medicine group decreased significantly(P<0.01)(P<0.01).Compared with the blank group,the body weight of the other three groups decreased significantly,and the results were statistically significant.The results showed that there was significant difference between the two groups Differences(P<0.01);3.The consumption rate of sugar and water in each group was not statistically different.From the trend analysis,the preference rate of the sugar group in the young group was the highest,the model group was the second,while the traditional Chinese medicine group had lower preference rate Compared with the juvenile group,the preference rate of sugar and water was higher than that of the model group.Compared with the model group,the preference rate of the model group was further decreased after the adult model group,Continued to rise,higher than the adult group after the blank group of sugar water preference.4.The activity of the fluoxetine group was slightly lower than that of the traditional Chinese medicine group,and the activity of the two groups was higher than that of the untreated group high.After the drug treatment,the traditional Chinese medicine group and the western medicine group compared the model group in the activity of this area there is a clear contrast,with statistical significance.5.Central time and central area:Although the data were not statistically significant,the results showed that the overall trend of the data and the distribution of the activity group were similar.The results were blank group>flavored Sini powder group>fluoxetine group>model group trend.6.There were significant differences in the results of the rats in the model group and the blank group.There was a significant difference between the Chinese medicine group,the western medicine group and the model group.In the adult rats,compared with the model group,There was a significant difference between the two groups.The total distance of the fluoxetine group was significantly different from that of the blank group and the traditional Chinese medicine group7.Changes of cell morphology in hippocampal CA1 region of rats:no significant abnormality was observed in HE staining sections of adolescent blank rats;a small amount of neurons were deeply stained in adult sections;adult neurons were shrunk and deeply stained in adulthood;The number of neurons in the adult group was significantly higher than that of the control group(P<0.05).The number of glial cells in the adult group was significantly higher than that in the control group A large number of neurons in the nucleus pyknosis,cytoplasmic eosinophilic enhancement,local neuronal loss;microglia proliferation,a small amount of eosinophil infiltration;fluoxetine group:adolescent individual neurons atrophy,cell body deep staining;A small amount of neurons atrophy,deep staining,cell body slender;adult more neurons atrophy,deep staining,cytoplasmic Nissl body reduction,a small amount of glial cell proliferation;addicted Sini powder group adolescents see individual nerve Atrophic,deep staining;adult neurons atrophy,deep staining,nucleus,cytoplasm boundaries unclear;adult after a small amount of neurons atrophy,deep staining.8.5-HT1AR mRNA expression:5-HT1AR mRNA expression in each group was not statistically significant(P>0.05).From the trend analysis,the expression of 5-HT1AR mRNA in adolescents was significantly higher The expression level of fluoxetine was lower than that of the model group,and the expression of mRNA was similar to that of the young group.The expression level of the modified Sini powder group was slightly lower than that of the blank group In addition to the blank group,the expression of each group was close to each other,and the trend was still in the model group>fluoxetine group>flavored Sini powder group.Conclusion1.Modified Sini Powder for the use of chronic unpredictable stress modeling 21 days of pediatric adolescent depression in the general state of depression,decreased interest and activity and explore the behavior of a certain improvement.Analysis from the overall trend,add the Sini Powder can be a certain extent to improve the depression caused by depression in rats.But can not reverse the decline in weight loss caused by depression.At the same time,combined with adult and adult outcomes suggest that pediatric adolescents have been subject to chronic unpredictable stimulation of the model group,after adult and adult stimulation,depression further deepened.2.Modified Sini Powder can improve the damage of hippocampal CA1 neurons and to some extent reverse the change of hippocampal neuronal cell structure caused by depression,which has a protective effect on hippocampus with stress injury.3.Modified Sini Powder can improve the protein expression of 5-HT1AR and 5-HTT in hippocampus CA1 region.This study suggests that Jiawei Sini Powder can effectively improve the expression of postsynaptic 5-HT1AR,regulate the 5-HT1AR negative feedback mechanism of postsynaptic,and enhance the function of 5-HTT transporters in synaptic reabsorption.In summary,the previous research group confirmed that a large number of research results confirmed that Jiawei Sini powder for young children with chronic stress depression model rats and mother and child separation depression model rats have therapeutic effect.Pediatric adolescence in the liver often have this special physiological and pathological conditions,if the breast is improper or by external stimuli,it is easy to develop into emotional abnormalities,from the liver treatment.