| Dracaena Vandelli ex Linnaeus is a rare tropical medicinal plant and it is also a famous leaf ornamental plant.According to the records of the Lisu Bay leaves,the leaves of Dracaena cambodiana have similar medical effect as Sanguis Draxonis.There have been some pharmaceutical companies developing the leaves of Dracaena cambodiana into a dai medicine baimaigashai and put them on the sale,market feedback and preliminary pharmacological experiments show that baimaigashai have a significant effect on diabetes,But the hypoglycemic mechanism of Dracaena cambodiana leaves has not been revealed.In order to clarify the chemical nature of the effective components of the leaves of Dracaena cambodiana,and to provide a scientific basis for further development of the drug.This study focuses mainly on the systematic research into its water-soluble active ingredients.In this research,the leaves of Dracaena cambodiana were used as raw material,the aqueous extract of hypoglycemic activity is extracted by hot water,then precipitated raw sugar by ethanol,the yield was 8.59%.The reagent for the removal of free protein was Sevag(the optimal volume ratio of chloroform and n-butanol is 5:1),the sugar retention rate is 98.11%,and the protein removal rate was 23.83%.The best decolorization material is A-722 MP macroporous strong base anion exchange resin,resin retention rate was up to 90.92%,the decolorization rate was 73.03%.The hypoglycemic active substance tracing model was established by using L6 rat skeletal muscle myoblasts and the active ingredients were separated and purified under the guidance of this model.The glucose content of the culture solution was detected by the glucose oxidase-peroxidase method.With DEAE-52 cellulose column chromatography,the deionized water and sodium chloride solutions with different concentration gradient(0.1mol/L,0.3 mol/L,0.6 mol/L,1mol/L)gradient were used as eluent,phenol-the sulfuric acid method was used to trace and draw the elution curve.Four sugar components QLZI?QLZII?QLZIII?QLZIV were gotten,and the main components QLZI possesses the most active hypoglycemic characteristic.Then the QLZI is purified by Sephacryl S-100 HR gel,using deionized water as eluent,phenol-sulfuric acid method and coomassie brilliant blue method respectively tracer elution curve drawing and sugar and protein,obtain sugar-protein mixture LXS,fructan ligand LZS,active protein LSP,all of them have hypoglycemic activity.The activity will improve when the quality of LZS and LSP are mixed in the proportion of 1:1.The LZS is determined and analysized by UV spectrum,IR spectrum,mass spectrometry,NMR spectra and HPAEC-PAD,the result show that it is a compound of Polysaccharide ligand containing 1.45% citric acid and its main Monosaccharides are Fructose,glucose,galactose and Arabinose.According to some relating references and the relating data in the SciFinder database,as far as we know that the LZS is a new kind of compound.The molecular size of active protein,LSP,is approximately 13000 Da determined by SDS-PAGE.This research has confirmed that these sugar and binding proteins are an important type of active ingredient in dai medicine baimaigashai,it provides a theoretical and experimental basis for diabetes-curing pharmacological mechanism and the development of medicines. |
PDF Full Text Request