Experimental Study On The Role Of IL-6 In Hydatidosis-induced Allergic Reaction

Objective: To study the changes of IL-6 and its related cytokines during the infection stage of Echinococcus granulosus and its allergic reaction,and to investigate the immune response of IL-6 in mice with hydatid-induced anaphylactic shock.Methods: The protoscolex of cystic hydatid was pre-cultured in vitro to produce microcapsules with a diameter of 200-300 um.In the Hydatid model group,50 microcapsules were injected intraperitoneally into each BALB/c mouse to establish a model of mice infected with Echinococcus granulosus.The same dose of PBS was injected into the abdomen of all mice in the control group.Three months after infection,allergic mice were injected intraperitoneally with 0.1ml/10 g crude granules of hydatid to induce anaphylaxis.The same dose of PBS was injected intraperitoneally into hydatid model group and blank control group.The levels of IL-6-related inflammatory factors and immunoglobulin in serum of mice were detected by flow cytometry microbead array.Results: Hydatid cysts were found in the abdominal cavity and liver surface of model group and allergic group.The serum levels of IL-6,IL-17 A and TNF-α in allergic group were higher than those in blank control group and model group(P<0.05).Conclusion: The serum levels of IL-6,IL-17 A,TNF-α and IL-10 in the model group increased.After sensitization,the levels of IL-6,IL-17 A and TNF-α continued to increase,while the levels of IL-10 decreased.IL-6,as a proinflammatory factor,is significantly elevated when hydatid anaphylaxis occurs,suggesting that it plays an important role in the occurrence of anaphylactic shock.