The Effects Of SDG On The Proliferation And Differentiation Of Osteoblasts And The Expression Of OPG MRNA

Objective:Along with the social civilization progress,oral care also gradually to get people’s attention,including dentition defect and tooth loss is one of the factors affected oral health and function.Due to the requirement of people is increasing,the conventional traditional tooth repair way already can not meet the needs of patients.In bone combined with the mature of the theory of context,oral planting to missing teeth and denture repair technology not only obtained the rapid development,but also gradually accepted by people.But also brings a lot of related problems,such as not all of the patients are suitable for growing tooth repair,including osteoporosis is an important factor contribute to the success of dental implants.Postmenopausal women,in particular,has been confirmed due to the changes of estrogen levels,can lead to the occurrence of osteoporosis,which is grown in the clinical effect in the women after repair success rate.The secoisolariciresinol diglucoside(SDG),in the prevention and treatment of women with postmenopausal osteoporosis caused by clinical efficacy has been confirmed,can improve the patients serum calcium content and bone mass in the body,increase the degree of bone is sensitive to the hormone by the thyroid gland,promote the formation of new bone matrix,has the significant effect on the restraint of bone loss.As one of the female hormones of the plant,it has a higher degree of similarity with human estrogen,which is derived from flaxseed,which is usually about 0.9%～1.5%of the seed.In this experiment,the osteoblasts were cultured in vitro by secoisolariciresinol diglucoside to detect and analyze the effects of its expression,proliferation and differentiation on the genes related to osteoblasts.Method:In vitro cultured osteoblast cells were cultured in vitro and the effects of apoptosis on the proliferation and differentiation of osteoblasts and the expression of OPG gene were evaluated.This study firstly has been configured with different concentration gradient of the secoisolariciresinol diglucoside to cultivate the same number of osteoblast,at a certain time point,determined by MTT colorimetric method is used to observe the cell adhesion and proliferation;The differentiation of osteoblasts was analyzed by ALP activity detection method.Rt-qpcr was used to detect the gene expression of the osteoprotegerin.Results:1.determined by MTT test show that the different concentration gradient respectively the SDG broth culture osteoblast 1 day,2 days,3 days,compared with the blank group,the result shows that 1 d and 2 d point in time,the drug concentration of 10-5 moL/L culture of osteoblast proliferation is restrained,with statistical significance(P<0.05),the 3 d point detection data showed no statistical significance;The drug concentration was 10-6moL/L and 10-9moL/L medium,and the changes of osteoblasts were not obvious,and there was no statistical significance.Concentration is 10-7 moL/L and 10-8 moL/L culture of osteoblast proliferation,increase trend,including osteoblasts in concentration is 10-7 moL/L medium,proliferation,the most obvious difference is statistically significant(P<0.05).2.alkaline phosphatase(ALP)activity determination showed that concentration of 10-7mol/L to 10-8 mol/L culture,ALP activity compared with blank control group,with statistical significance(P<0.05),especially the concentration of 10-7 mol/L ALP activity in the cultures of the highest.In the concentration of 10-5 mol/L medium,the relative activity of ALP and blank group decreased significantly,which was statistically significant(P<0.05).The concentration was 10-6moL/L and 10-9moL/L medium,and the activity of ALP did not change significantly.3.Rt-qpcr was used to detect the gene expression of protectin in 3 days,compared with ck,according to the results of drug concentrations is 10-5moL/L,osteoprotegerin expression quantity showed a trend of lower,but there was no statistically significant difference;The drug concentration was 10-8moL/L and 10-9moL/L.When the concentration of drugs was 10-6 mol/L and 10-7 mol/L,the expression trend increased significantly,which was statistically significant(P<0.05).Conclusion:In vitro culture of osteoblasts was cultured in vitro with different concentration gradients,and the control effect was observed on the cells with a high concentration of SDG(10-5 mol/L).Low concentration(10-6 moL/L,10-7moL/L,10-8 moL/L)and numbness,lignans cultures ofosteoblast proliferation rate,to raise trend and OPG gene expression quantity to increase,particularly in the SDG concentration is 10-7 moL/L,osteoblast proliferation and raise the volume of OPG gene expression is obvious;When the concentration of drug is 10-9 mol/L,it may not be obvious due to the low concentration.The results showed that the SDG was effective in promoting the proliferation and differentiation of osteoblasts.