Study On Molecular Mechanism And Function Of Foxc2-targeted MiR-199a-5p In EMT Of Gastric Cancer

Background:Forkhead box protein C2(FOXC2)is an important regulatory factor in the process of epithelial-mesenchymal transition(EMT),this critical process is frequently activated in tumor progression and metastasis.Aim:We take gastric cancer as the research object to explore the molecular mechanism of miRNA-199a-5p targeting the key regulatory molecules FOXC2 of EMT in gastric cancer,determine which miRNAs have the capable of regulating transcription factor.Methods:Real-time RT-PCR and Western blot were used to examine the FOXC2 expression in the tumor and its adjacent normal tissues.Real-time RT-PCR was applied to determine the level of miR-199a-5p in the patient organization.Then we used the experimental technique of transfection to overexpression and knockdown the miR-199a-5p in the SGC-7901.Effects of miR-199a-5p on SGC-7901 cells migration and invasion were accounted.Western blot was used to determine associated marker protein of EMT.Dual Luciferase Reporter Assay was used to prove whether FOXC2 was a target of miR-199a-5p or have other indirect action relationships.Results:Decreased miR-199a-5p and Increased FOXC2 expression coexisted in tumor tissue in patients with gastric cancer.Overexpression of miRNA-199a-5p can inhibit the invasion and migration of SGC-7901 and knockdown of miRNA-199a-5p can reduce the expression of E-cadherin and improve the expression of Vimentin.Dual Luciferase Reporter Assay confirmed that miR-199a-5p could directly target FOXC2 through binding to its 3’-UTR.Conclusions:This study indicated that the down-regulation of miR-199a-5p can induce FOXC2 up-regulation,and FOXC2 was a target of miR-199a-5p,this effect may be associated with the invasion and migration of tumor,via activating the EMT-related signaling.