Effects Of Timeless Interference On Apoptosis,Cycle And Expression Of β-catenin In SGC-7901 Gastric Cancer Cells

Purpose:To explore effects of Timeless interference on apoptosis,cycle and expression of β-catenin in SGC-7901 gastric cancer cells.Methods:The siRNA of Timeless was synthesized and transfected into SGC-7901 cells by Lipofectamine? 3000.Then the most efficient Timeless siRNA was chosen to make the Timeless interference group.Three experiment groups were set: blank control、siRNA control group and siRNA-Timeless group.The expression of Timeless and β-catenin mRNA and protein was measured by Realtime PCR and western blot.Cell viability was measured by MTT.Cell apoptotic rate and cell cycle was detected by flow cytometry.Results:The transfection efficiency of Timesless-siRNA-2 was the highest via Realtime PCR and western blot.Compared with blank control group and siRNA control group,the expression of Timeless and β-catenin mRNA and protein was down-regulated in siRNA-Timeless group(P<0.01),cell viability was decreased(P<0.01),cell apoptotic rate was increased(P<0.01),cell cycle was made arrested at S phage(P<0.01).Conclusions:Interference with Timeless expression could significantly induce apoptosis of SGC-7901 gastric cancer cells,and inhibit cell proliferation,which related to down-regulation expression of β-catenin expression.