Voltage-gated Sodium Channel Nav1.5 Promotes Proliferation Migration And Invasion Of Oral Squamous Cell Carcinoma

Objective Oral squamous cell carcinoma(oral squamous cell carcinoma,OSCC)is one of the most common high malignant tumors in human oral and maxillofacial region,which is highly metastatic,accounting for more than 90% of oral and maxillofacial malignant tumors.The protein voltage-gated sodium channel Nav1.5 is highly upregulated in various types of cancer and in general,promotes cancer cell invasiveness and metastatic progression.A previous study had found that Nav1.5 was highly expressed in poorly differentiated oral squamous cell carcinoma(OSCC).However,whether Nav1.5 enhances invasiveness and metastasis of OSCC is still unknown.Methods Western bolt,Q-RT PCR,si RNA transfection and CCK-8,transwell invasion and migration techniques were used to detect the effects of Nav1.5 on the proliferation,migration and invasion of oral squamous cell carcinoma cells.Results In this study,we found that Nav1.5 was highly expressed cell lines.Downregulation of Nav1.5 expression by its specific si RNA reduced proliferation,migration and invasion of oral squamous cell carcinoma HSC-3 cells.In contrast,EGF treatment induced Nav1.5 expression and promoted proliferation,migration and invasion of HSC-3 cells.Importantly,the Nav1.5 inhibitor tetrodotoxin(TTX)significantly attenuated the effect of EGF-induced Nav1.5 expression on proliferation,migration and invasion of HSC-3 cells,and EGF could significantly enhance the proliferation,migration and invasion of HSC-3 cells transfected with si RNA.Conclusions Taken together,our results indicate that Nav1.5 promotes proliferation,migration and invasion of oral squamous cell carcinoma.