Improvement Effects Of Bisdemethoxycurcumin On SH-SY5Y Cells Injured By Rotenone Through JAK2/STAT3 Pathway

[Objective]This study was to investigate the mechanism by which bis-demethoxycurcumin(BDMC)improves the damage effects of rotenone on SH-SY5 Y cells,and further clarify whether BDMC can improve the damage effects of rotenone on SH-SY5 Y cells by activating JAK2/STAT3 pathway.[Methods]1.Establishing the cell model of PD by rotenone-induced SH-SY5 Y cells.The optimal concentration of rotenone was determined by MTT assay for the viability of SH-SY5 Y cells.The effects of rotenone on oxidative stress in SH-SY5 Y cells were observed by the activity of superoxide dismutase(SOD)and glutathione(GSH).2.Investigating the protective effect of BDMC on SH-SY5 Y cells injured by rotenone.The optimal concentration and protective effects of BDMC were determined by MTT assay for the viability of SH-SY5 Y cells.The activity of SOD and GSH were measured to observe the effects of BDMC on the improvement of oxidative stress on SH-SY5 Y cells injured by rotenone.3.Investigating the effect of BDMC on JAK2/STAT3 pathway on SH-SY5 Y cells injured by rotenone.Western blotting was used to measure the expression of p-JAK2 and p-STAT3 to observe the effect of BDMC on the activation of the JAK2/STAT3 pathway on SH-SY5 Y cells injured by rotenone.4.Investigating the effect of JAK2/STAT3 pathway inhibitor AG490 on the protective effect of BDMC on SH-SY5 Y cells injured by rotenone.Western blotting was used to measure the expression of p-JAK2 and p-STAT3 to observe the inhibitory effect of AG490 on JAK2/STAT3 pathway.MTT assay was used to observe the effect of AG490 on improving cell viability of BDMC on SH-SY5 Y cells injured by rotenone.The activity of SOD and GSH was measured to observe the effect of AG490 on the anti-oxidative stress effect of BDMC on SH-SY5 Y cells injured by rotenone.5.Investigating the effect of STAT3 inhibitor S3I-201 on the protective effect of BDMC on SH-SY5 Y cells injured by rotenone.Western blotting was used to measure the expression of p-STAT3 to observe the effect of S3I-201 on the negative expression of STAT3.MTT assay was used to observe the effect of S3I-201 on improving cell viability of BDMC on SH-SY5 Y cells injured by rotenone.The activity of SOD and GSH was measured to observe the effect of S3I-201 on the anti-oxidative stress effect of BDMC on SH-SY5 Y cells injured by rotenone.[Results]1.Rotenone can induce SH-SY5 Y cytotoxicity to decrease cell viability and oxidative stress lead to decreased of activities GSH and SOD.2.BDMC can improve the cell viability of SH-SY5 Y cells injured by rotenone and improve the activity of GSH and SOD to improve oxidative stress.3.BDMC can activate the JAK2/STAT3 pathway on SH-SY5 Y cells injured by rotenone and up-regulate the expression of p-JAK2 and p-STAT3.4.JAK2/STAT3 pathway inhibitor AG490 can reverse the effect of BDMC on the cell viability and anti-oxidative stress of SH-SY5 Y cells injured by rotenone.5.STAT3 inhibitor S3I-201 can antagonize the improvement of BDMC on the cell viability and anti-oxidative stress of SH-SY5 Y cells injured by rotenone.[Conclusion]Improvement effects of bisdemethoxycurcumin on SH-SY5 Y cells injured by rotenone through JAK2/STAT3 pathway.