The Effect Of MiR-221/222 On Intrahepatic Cholestasis Of Pregnancy Through Promoting The Apoptosis Of HTR-8/Sveno Cells

Objective: In order to confirm the miR-221/222 play an important part in the pathogenesis progress of Intrahepatic cholestasis of pregnancy,we have analyzed the effect of miR-221/222 on the proliferation,apoptosis and relevant protein expressions of trophoblast cell(HTR-8).Methods: The compound miR-221/222 mimic were synthesized by a chemical synthesis method,then wrapped with the Lipofectamine 3000 and transfected into HTR-8 cells.miR-221/222 were quantified with Real-time Quantitative polymerase chain reaction(RT-qPCR).CCK8 was used to study the effect on cell proliferation and cell apoptosis was detected by flow cytometry.The protein expression of Bcl-2 was assayed by western blot.Results: With the treatment of miR-221/222 mimic,the expression of miR-221/222 in HTR-8 increased.The CCK8 and flow cytometry result shows that the apoptosis of HTR-8 cell with the miR-221/222 mimic transfection increased compared with control group.Protein expression of Bcl-2 in miR-221/222 mimic group decreased compared with control group.Conclusion: The results show that miR-221/222 mimic increase apoptosis,while decrease the protein expression of Bcl-2 in HTR-8 cell.These findings indicate that miR-221/222 mimic may enhance the ability of apoptosis through regulating Bcl-2,leading to dysplasia and dysfunction of placenta,then contributing to the pathogenesis of ICP.