| Background:In recent years,with the change of people's lifestyles,the advancement of medical science,the popularization of screening medical examinations and the update of examination methods,the incidence of breast cancer has also increased year by year,and the age of onset has also tended to be younger.At present,the incidence of breast cancer is 11.6%,and it has become one of the most common malignant tumors among women in the world.Its mortality rate ranks fourth among all malignant tumors of women,seriously endangering women's health.The breast is the target organ of various endocrine hormones,including estrogen,progesterone and prolactin.Thyroid hormone also acts on the breast.As early as 1896,Beatson proposed the use of thyroid hormone in breast cancer patients to achieve the purpose of treatment.Since then,scholars have carried out some studies to explore the relationship between thyroid hormones and breast cancer,mainly focusing on epidemiological studies comparing the levels of thyroid hormones in breast cancer and healthy people and the relationship between thyroid hormones and the risk of breast cancer.There are few studies on the relationship between hormone levels and clinical characteristics of breast cancer.Objective:The purpose of this study was to explore the relationship between thyroid hormone levels and clinical characteristics of breast cancer patients,and to conduct cytological experiments based on the results.Methods:1.Collection of clinical data:This study retrospectively collected medical records of breast cancer patients hospitalized in the Shandong Provincial Hospital from November 2017 to June 2019,collecting the patient's age,menstrual status,medical history,medication history,imaging examination,surgical records,pathological reports,chemotherapy records,laboratory test results,etc.2.Analysis of clinical data:Patients were grouped according to age,menstrual status,tumor size,metastasis,TNM stage,pathological grade,estrogen receptor,diabetes prevalence,surgery and chemotherapy,tumor marker levels.Statistical analysis was performed on the differences of thyroid hormone levels in different clinical characteristics of patients.Descriptive statistical methods are used to summarize the characteristics of patients.Qualitative data is described by relative number.Quantitative data is tested for normal distribution by Kolmogorov-Smimov method.The quantitative data conforming to the normal distribution were described as mean ± standard deviation,the T-test,the one-way ANOVA and covariance analysis were used to compare the mean between groups.The quantitative data of non-normal distribution is expressed by median(quartile distance),and the rank-sum test is used for inter-group comparison.3.Cell proliferation experiment:CCK-8 method was used to detect the effect of different concentrations of T3 on the proliferation of MDA-MB-231 cells.4.Cell scratch test:To observe the effect of different concentrations of T3 on the migration ability of human breast cancer MDA-MB-231 cells.Results:1.In 142 female breast cancer patients,FT3 levels were higher in the distant metastasis group than that in the non-metastasis and the local lymph node metastasis group(P?0.001),FT4 levels were higher in the non-metastatic group than that in the local lymph node metastasis group(P=0.001).Taking age,menstrual status,estrogen receptor,diabetes,surgery and chemotherapy as covariables,analysis the correlation between patients' metastasis status and thyroid hormone levels,found that FT3 levels were higher in the distant metastases group than that in the non-metastatic and the local lymph node metastasis group(P?0.001),FT3 level was higher in the non-metastatic group than in the local lymph node metastasis group(P?0.001).FT4 level in the local lymph node metastasis group was lower than that in the non-metastatic and the distant metastasis group(P?0.001).The FT3 level of patients who had received surgical treatment was higher than that of patients without surgery(P?0.05).FT3 levels of patients who have received chemotherapy are higher than those without chemotherapy(P?0.001).FT4 levels of patients who have received chemotherapy are lower than those without chemotherapy(P?0.05).2.T3 can promote the proliferation of MDA-MB-231 cells.3.T3 has an inhibitory effect on the migration ability of MDA-MB-231 cells.Summary and conclusions:The metastasis of breast cancer is related to higher free triiodothyronine levels.Both surgery and chemotherapy may affect thyroid hormone levels.T3 may promote the proliferation and inhibit migration of human MDA-MB-231 cells. |
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