| Objective: Using male KM mice as experimental subjects,an obstructive jaundice(OJ)model was constructed to clarify the intervention effect of moderate-intensity aerobic treadmill exercise on the recovery period of OJ intestinal mucosal barrier injury mice and to explore its mechanism of action.Methods: Fifty male KM mice were randomly divided into 5 groups:sham operation group(S group),model group(M group),aerobic exercise group(TM group),sodium hydrosulfide(NaHS)combined exercise group(NT group),DL-propargylglycine(PAG)joint exercise group(PT group).M,TM,PT and NT group mice are all constructed by this group,a safe and effective surgical line at right angles to suspend the common bile duct method to construct OJ mouse model,The mice in group S only made an incision in the abdomen and sutured it as a blank control group.Five days after the suspension of the common bile duct was lifted,the TM,NT and PT groups underwent weightless training(75% VO2max)with a slope of0% and a speed of 12 m / min for 60 minutes every day,6 days a week for a total of 6 weeks;In the PT and NT groups,PAG(40mg / kg)and NaHS(50μmol / kg)were injected intraperitoneally,6 times a week,where NaHS is the donor of H2 S,increasing the content of H2 S in the body,PAG is aninhibitor of H2 S synthase Inhibit the production of H2 S.After the experiment,the blood was collected by anesthesia,the animals were sacrificed and the intestinal tissue was retrieved.Spectrophotometry was used to detect H2 S in the serum and ileum tissues of each group of mice;HE staining was used to observe the pathological changes of the intestinal mucosa morphology of each group of mice;rate method was used to detect the content of serum diamine oxidase(DAO);ELISA Detection of serum D-lactic acid(D-LA)content;immunohistochemical detection of HMGB1,TLR4,NF-κB protein expression levels in ileal tissue;fluorescent quantitative PCR detection of HMGB1,TLR4,NF-κB gene expression levels in ileal tissue.Results:(1)HE staining showed that the villous morphology of group S was normal;the intestinal mucosa of group M was atrophied and the villi were broken;the structure of intestinal mucosa of mice in TM group was improved;The intestinal mucosa of the mice in the NT group was basically normal;the villous tissue in the PT group was shorter,the arrangement was disordered,and the basal inflammatory cells infiltrated.(2)H2S content in serum and ileum tissue: H2 S content in TM group was higher than that in M group and PT group(P <0.05,P <0.01),and NT group was higher than that in TM group(P <0.01).(3)Intestinal mucosal barrier function evaluation index test results: The serum DAO and D-LA contents in group S were the lowest,and the content in group M was the highest.The content of TMgroup was lower than that of M group and PT group(P <0.01).Compared with TM group,NT The content of DAO and D-LA in the group decreased(P <0.01).(4)Real-time PCR results showed that compared with the M group,the expression levels of HMGB1,TLR4,and NF-κB mRNA in the TM group were significantly reduced(P <0.05,P <0.01).Compared with the TM group,the expressions of TLR4 and NF-κB mRNA in the NT group were significantly reduced.The levels decreased(P <0.05),and the levels of HMGB1 and NF-κB mRNA in the PT group increased(P <0.01,P<0.05).(5)The results of immunohistochemistry showed that HMGB1,TLR4,and NF-κB were lowly expressed in the S group.Compared with the M group,the expressions in the TM group were decreased(P <0.01,P<0.05).Compared with the TM group,the expression of each factor in the NT group decreased(P <0.01,P <0.05),and the expression of the PT group increased(P <0.05).Conclusions: Moderate-intensity aerobic exercise can significantly alleviate the damage of intestinal mucosal barrier function in the recovery period of OJ mice.The mechanism may be related to the aerobic exercise that promotes the production of endogenous H2 S and further inhibits the HMGB1 / TLR4 / NF-κB signaling pathway. |
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