Relationship Between Rs2665936 Polymorphism Of TRAPPC9 Gene And Lung Cancer Susceptibility

Objective: Lung cancer is one of the most common cancers in the world,causing more deaths each year than any other cancer.In recent decades,both men and women have high morbidity and mortality.In China,the mortality rate of lung cancer has also remained high.By comparing and analyzing the incidence and mortality of lung cancer in China and the world,the standardized incidence and mortality rate of lung cancer in the world are both higher than the average level of the world.Therefore,lung cancer has become one of the major diseases endangering the life and health of Chinese residents.Transport protein particle complex 9(TRAPPC9),like yeast Trs120,is an essential and unique subunit of the TRAPP II complex that regulates the withdrawal of the trans-golgi apparatus.The trans-golgi network is crucial to the development of cancer,and autophagy is also a key process for tumorigenesis.TRAPPC9 regulates trans-golgi body function and autophagosome formation during cancer development.Some studies have confirmed the existence of TRAPPC9 transcription in various cell lines and tumor tissues,and clinical studies have also reported the potential association of TRAPPC9 transcription with human breast cancer,colon cancer,osteosarcoma and lymphoma,but there have been no studies on the relationship between TRAPPC9-rs2665936 and lung cancer susceptibility in the Chinese population.This study is the first to explore the relationship between TRAPPC9-rs2665936 polymorphism and lung cancer susceptibility in the Chinese population using case-control methods.Methods: This study used a hospital-based case-control study to genotype TRAPPC9-rs2665936 in 640 lung cancer patients and 699 healthy control samples.SPSS23.0 software was used for statistical analysis.All statistical tests were two-sided probabilistic tests,and P<0.05 was considered statistically significant.The T test and chi square test were used to compare the differences in age and sex distribution between the case group and the control group,and Logistic regression was used to calculate the OR value and its confidence interval.Result: A case-control study showed that the relationship between the genetic polymorphism at the TRAPPC9-rs2665936 locus and the susceptibility to lung cancer was statistically significant.Compared with the AA genotype population,the risk of lung cancer in people with the GG genotype was 1.943 times(adjusted OR=1.943,95% CI=1.361-2.774,P<0.001),in the dominant model(adjusted OR=1.375,95% CI=1.093-1.730,P=0.007),in the recessive model with AA genotype and heterozygous mutant AG as reference(adjusted OR=1.696,95%CI=1.224-2.350,P<0.001),there is a significant difference between the control group and the control group,that is,there is also an increased risk of lung cancer.The results of stratified analysis showed that the TRAPPC9-rs2665936 locus was associated with the risk of lung adenocarcinoma,a genetic model(GG vs AA: adjusted OR=2.174,95% CI=1.485-3.183,P <0.001;AG + GG vs AA: adjusted OR=1.444,95% CI=1.121-1.861,P=0.005;GG vs AA + AG: adjusted OR=1.880,95% CI=1.330-2.657,P<0.001)all increased the risk of lung cancer.Also in the age stratification analysis,the results are significant;in the gender stratification,the statistical results for women are significant,and there is no statistical difference for men;for the smoking exposure stratification,the statistical results of non-smoking are significant,and there is no statistical difference in smoking.In the final interaction analysis,there was no additive or multiplicative interaction between the genetic polymorphism at the TRAPPC9-rs2665936 locus and the effect of smoking exposure on lung cancer risk.Conclusion: The genetic polymorphism at the TRAPPC9-rs2665936 locus is associated with lung cancer susceptibility and affects the risk of lung cancer.2.The genetic polymorphisms at the TRAPPC9-rs2665936 locus in different ages,genders,pathological tissue types,and smoking exposures have different relationships with lung cancer susceptibility.3.During the occurrence and development of lung cancer,no interaction was found between site polymorphisms and smoking exposure.