The Effect Of Dioscin From Dioscorea Nigra Extract On The Expression Of Inflammatory Factors In The Sciatic Nerve Of PDPN Mice

Purpose:To investigate the effect of dioscin of dioscorea chinensis extract on expression of NF-κB,pro-IL-1β,NLRP3 and IL-1β inflammatory factors in sciatic nerve of mice with painful diabetic peripheral neuropathy.Material and method:Eighty-three SPF male C57BL/6 mice(20-25 g)were selected.Mice were reared in a SPF environment at 23 ± 0.5℃,12 h cycle light.After screening the normal group by random number table method,the remaining mice were fed with high-fat feed for 8 weeks.After 12 hours without food,mice were injected intraperitoneally with 70 mg / kg 1% streptozotocin solution for two consecutive times to induce diabetes.After 6weeks of normal feeding,the MWT was detected,and the blood glucose was greater than 16.7mmol / L,and the MWT decreased by 50% compared with the basic value,which was the PDPN model.The model mice were randomly divided into model group and high-dose dioscin group,low-dose dioscin group,α-lipoic acid group,12 in each group.Detect the behavior,blood glucose,and body weight changes of mice at 0w before and 2w,4w,6w,and8 w after administration,as well as the pain threshold(mechanical pain threshold,heat pain)of mice at 0w before and 4w,8w after administration Threshold)change.At the 8th week,the material was taken,and HE staining was used to observe the pathological changes of sciatic nerve tissue in mice,and the protein expression of inflammatory factors NF-κB,pro-IL-1β,NLRP3 and IL-1β in sciatic nerve tissue of mice with painful diabetic peripheral neuropathy was detected by Western Blot technology.Results:1.The mice in the model group were drinking more,eating more,drinking more urine,curling down the back of the bow,losing energy,dark yellow hair,and poor gloss.The status of the mice in the treatment group was significantly better than that of the model group.2.During the whole experiment,except for the normal group,the weight of mice in the other groups had a significant downward trend(P <0.01);The weight of the treatment group decreased slowly compared with the model group(P <0.01).3.During administration,the blood glucose of mice in each treatment group showed a downward trend(P <0.01);The blood glucose decline trend of the high dose group of dioscin and the α-lipoic acid group was significantly better than that of the low dose group of dioscin.4.The mechanical pain threshold of mice in the normal group was significantly higher than that in the other groups(P <0.01);In the course of treatment,the mechanical pain threshold of mice in each treatment group had a significant upward trend compared with the model group(P <0.01).5.The heat pain threshold of mice in the normal group was significantly higher than that in the other groups(P <0.01);During the course of treatment,the heat pain threshold of the mice in each treatment group had a significant upward trend compared with the model group(P<0.01).6.Compared with the normal group,the expression of NF-κB,pro-IL-1β,NLRP3,and IL-1βprotein in the sciatic nerve tissue of the model group all showed a significant upward trend(P<0.01);After treatment,compared with the model group,the expression of NF-κB,pro-IL-1β,NLRP3,and IL-1β protein in the sciatic nerve of each treatment group had a certain tendency to decrease(P <0.01).It is suggested that dioscin of dioscorea chinensis extract can reduce the expression of NF-κB,pro-IL-1β,NLRP3 and IL-1β protein in sciatic nerve of PDPN mice.7.The sciatic nerve fibers of the normal group were intact,well-arranged and evenly distributed.The sciatic nerve fibers in the model group were disordered,unevenly distributed and degenerated and ruptured in multiple places,and fibrous space increased;Compared with the model group,the sciatic nerve fibers of the mice in each treatment group showed less fibrous interstitial edema.It is suggested that dioscin of dioscorea chinensis extract have certain therapeutic effects on the pathological changes of sciatic nerve fibers in PDPN mice.Conclusion:Dioscin of dioscorea chinensis extract can alleviate the weight loss trend of PDPN mice,and can also reduce the blood sugar of PDPN mice,and can down-regulate the expression of inflammatory factors NF-κB,pro-IL-1β,NLRP3,and IL-1β in the sciatic nerve of painful diabetic peripheral neuropathy mice,and reduce edema between the sciatic nerve fibers,reduce the inflammatory response,and delay the process of nerve damage.