Study On The Expression Of Pathological Related Indexes Of Internal Hemorrhoids

Objective:At present,there are few pathological studies on internal hemorrhoids,so the pathophysiological mechanism of its occurrence and development is not clear.Therefore,we designed an experiment to observe the expression of histopathological indexes of internal hemorrhoids by pathology and immunohistochemistry,and to explore the pathogenesis and related factors of internal hemorrhoids,and whether there is a certain rule between TCM syndrome type and clinical stage.It also provides the basis for the clinical treatment of internal hemorrhoids.Methods:All the cases in this study were from the internal hemorrhoids patients who were hospitalized in the anorectal Department of the Second Affiliated Hospital of Henan University of traditional Chinese medicine from January 2017 to February2019 and operated on simultaneously.We use the hospital information management system to extract all clinical information of patients,and make statistics of their gender,age,diagnosis,clinical stage of internal hemorrhoids,TCM syndrome type,pathological diagnosis and other information.Combined with the above information,we can select 136 specimens of internal hemorrhoids from the pathological information database,which are in line with the research scope,and divide them into pathological group A,70 specimens,pathological group B,66 specimens.As a control,we need to select 2 cases of normal anal cushions tissue samples.The tissue was fixed by pathological routine method,then the material was taken and embedded in paraffin,the tissue was sectioned continuously and baked for standby.First,pathological changes of hemorrhoids in group A were observed under HE staining,statistical methods were used to analyze whether there were statistical differences in pathological changes of internal hemorrhoids of different TCM syndromes.In the second part,the pathological group B was treated by immunohistochemistry,and then the expression of CD34 and VEGFR2 in the internal hemorrhoids was observed to explore the microvessel density and angiogenesis level in the internal hemorrhoids.The images were observed and collected under electron microscope,and the data were analyzed by SPSS 19.0.Conclusion:1.Under HE staining,obvious pathological morphological changes were found in the internal hemorrhoids: the thickness of mucosa covered epithelium was uneven,continuous destruction,even erosion and ulcer;inflammatory infiltration was found in the tissues,mostly involving the mucosal layer and submucosa;sinus vascular structure was destroyed,and there was bleeding phenomenon of mass in the tissues,red blood cell aggregation and thrombosis were found in the vascular lumen,and new life was found in the tissues Blood vessels;the structure of Treitz muscle is loose,disordered,even broken and denatured;the stroma shows different degrees of edema,many dilated blood vessels can be seen in the stroma,and there are signs of recent or previous thrombosis,interstitial fibromuscular hyperplasia.2.Through the statistical analysis of the integral value of pathological indexes,there was no statistical difference in the pathological indexes of mucous membrane change,inflammatory infiltration and thrombus formation among different types of internal hemorrhoids(P > 0.05);3.In internal hemorrhoid tissue,there are obvious pathological changes in anal cushion mucosa,anal cushion blood vessel and Treitz muscle,but there is no statistical difference between these changes and the TCM syndrome type of internal hemorrhoids;CD34 and VEGFR2 in hemorrhoid tissue are positive in vascular endothelial cells and increase with the aggravation of internal hemorrhoid,indicating that there are new blood vessels in the process of hemorrhoid formation,and there is a positive correlation with the development of internal hemorrhoid.