| Objective(1)To investigate the differences between the HBV-related HCC and the non-HBV-related HCC in clinicopathology characters(including histopathological grade,liver capsule invasion,satellite nodules,microvascular invasion grade,cirrhosis and the expression of AFP and ki-67 etc.)and the clinical stage,in order to identify the effect of HBV in the development of HCC.(2)To investigate the expression of AR and ER in the HBV-related HCC,and the correlation of the expression and the clinicopathology characters and the clinical stage of the HBV-related HCC,in order to provide a new thought in the aspect of diagnose,therapy and prognosis etc.Methods(1)The medical records of patients who were diagnosed as hepatocellular carcinoma by pathology department of General Hospital of Ningxia Medical University from June 2016 to December 2020 were collected.And according to the clinical diagnosis of chronic hepatitis B,patients were divided into two groups:the group of HBV-related HCC and the group of non-HBV-related HCC.SPSS26.0 software was used to process and analyze the data of the clinicopathology characters of different groups.(2)Among the acquired cases,54 cases(25 females,29 males)of paraffin specimens of carcinoma and para-cancerous tissues of HBV-related HCC patients were randomly selected.And then using the immunohistochemistry to detect the expression of estrogen receptor(ER)and androgen receptor(AR)in the above samples.SPSS26.0 software was used to process and analyze the data of the clinicopathology characters of different groups.Results(1)Compared with non-HBV-related HCC patients,the age of HBV-related HCC patients(58.9±14.33 vs 55.12±10.1,p=0.041),clinical stage(advanced stage,25%vs 47.1%,p=0.009),recurrence and/or metastasis(5%vs 35.4%,p=0.000)etc.are statistically significant.Among HBV-related HCC patients,it is found that there is a statistical difference in viral load≤10~3copies/ml group and>10~3copies/ml group:tumor cell proliferation index(ki-67)(high index,14.0%vs27.8%,p=0.024)and clinical stage(advanced stage,41.3%vs55.7%,p=0.028).(2)In HBV-related HCC cancerous tissue,AR mainly expresses in the nucleus,and its expression is associated with the viral load(r=0.598,p=0.000);In HCC para-cancerous tissues,AR mainly expresses in the nucleus and plasma.(3)ER is mainly expressed in the nucleus of para-cancerous tissues.The expression of ER in HBV-related para-cancerous tissues is correlated with clinical stage(r=0.311,p=0.022).Conclusion(1)The influence of HBV DNA runs through the occurrence and development of HCC,and in order to achieve a better prognosis in patients with a background of HBV infection,it is necessary to conduct preoperative and/or postoperative antiviral therapy and inhibit HBV DNA replication,especially in HCC patients with high viral load and the postmenopausal female population.(2)AR mainly expresses in the nucleus of HCC cancerous tissue,and its expression is associated with the viral load,thus,it may be possible to predict the prognosis of patients by detecting the expression of AR in the cancer tissues of HBV-associated HCC patients and provide a basis for postoperative antiviral treatment.(3)ER is mainly expressed in the nucleus of para-cancerous tissues,thus,ER may be used as a marker to distinguish highly differentiated cancer tissues from para-cancerous hyperplasia and fibrosis liver tissues. |
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