Effects Of Plant Dietary Fiber On Intestinal Bacterial Diversity In Women With Dysmenorrhea

There are about 100 trillion microorganisms in the human body,about 10 times the number of human cells.These microorganisms are widely distributed in the digestive tract,respiratory tract,reproductive tract,urethra,skin and internal environment.The intestinal tract is also contain largest community of microorganisms.Human microorganisms are mainly including Firmicutes,Bacteroides,Proteobacteria,Actinomycetes,Clostridium and Verruca microflora.Among all the microorganisms,the abundance of Bacteroides and Firmicutes is the highest,which takes more than 90% of the total intestinal flora.Another important function of microorganisms is to maintain a symbiotic and antagonistic relationship in the intestinal tract,and also form a dynamically balanced microecosystem in the intestinal tract.When microorganisms are stimulated by the internal and external environment,the composition and structure of intestinal flora will change significantly,resulting in intestinal disorder,known as intestinal flora disorder.With the further studies on the relationship between intestinal flora and diseases,researchers have found that the disorder of intestinal flora is closely related to constipation,diabetes,hypertension,hyper lipidemia,neurological diseases,chronic metabolic diseases,autoimmune diseases,cancer and other diseases.Dietary fiber plays an important role in human health.Dietary intervention with fiber can regulate the secretory flora of intestinal short-chain fatty acids,and thereby improve the symptoms of type 2 diabetes.Dietary fiber has certain regulating effect on human intestinal flora.According to a large number of literatures,regulating intestinal flora can cure some chronic inflammatory diseases,and some chronic inflammation can cause menstrual cramps in women.However,no researchers have proved whether there is a certain correlation between menstrual cramps in women and intestinal flora through specific data.This experiment mainly explores the differences in intestinal flora structure between patients with dysmenorrhea and healthy people,and USES dietary fiber to intervene the intestinal bacteria of patients with dysmenorrhea to identify pathogenic bacteria and probiotics related to dysmenorrhea.Total 80 qualified samples were collected,comprising of 36 from the control group,22 from before and 22 from after the intervention of dietary fiber.Firstly,paired t-test analysis was performed on the questionnaire.Total DNA was extracted from fecal samples through experimental treatment,and then purified and amplified to obtain sample DNA.High-throughput sequencing technology was used for sequencing.According to OTU division,Alpha diversity analysis,Beta diversity analysis and LEf Se analysis,the results show that the intervention of dietary fiber plays a certain regulatory role in intestinal flora.The main bacteria causing dysmenorrhea were Collinsella aerofaciens,Fusobacterium and Blautia.Prevotella,Veillonella and Bacteroides coprophilus.These are the main bacteria those resistant to dysmenorrhea.S24＿7 and Parabacteroides distasonis play a key role in the improvement of dysmenorrhea.Dietary fiber does promote the adjustment of intestinal flora composition and species abundance to a healthy state.In this experiment,the intervention of dietary fiber has a regulating effect on the intestinal microflora structure and abundance of dysmenorrhea patients from a microscopic perspective,which can inhibit or reduce the species abundance of pathogenic bacteria groups,and stabilize the structure and abundance of normal probiotics to the abundance of normal healthy people.From a macro perspective,the pain index of dysmenorrhea patients decreased significantly,greatly improving the symptoms of dysmenorrhea patients.