A Novel Rodent Model Of Retinal Edema Induced By Photochemical Reaction And Its Relevant Application

Purpose: In this study,we established a rat retinal edema model to explore its relevant mechanism and application.Method: Rat retinal edema model was established by photochemical effects of532 nm laser after intravenous Erythrosin B infusion.Changes of rat reninal edema were observerd by fundus photography(FP),optical coherence tomography(OCT),fluorescein fundus angiography(FFA)at 0,1,2,4,7,14 days after intervention.Retinal VEGF concentrations were measured by enzyme linked immunosorbent assay(ELISA)at each time points.Models were intervened immediately by intravitreal injection of ranibizumab and conbercept to inhibit VEGF expression and observe retinal thickness changes.The rat retinal edema model was also used to verify the application of polypeptide ZY1.Results: Retinal edema and vascular leakage were observed obviously at 1,2,4 days after successful modeling,and retinal thickness increased notably at the same time.Retinal VEGF concentration peaked at 1 day,synchronized up with retinal thickness.After interventions,VEGF concentrations of ranibizumab group and conbercept group decreased at each time point(P < 0.01),and mean RT(P < 0.01)and vascular leakage in these group decreased as well.After intervention,comparing with model group,vascular leakage and retinal edema in ZY1 group also decreased,particularly at 2 day(P < 0.05).Conclusion:This retinal model induced by a photochemical reaction is reliable and repeatable.The up-regulated expression of VEGF is one of important mechanisms of retinal edema.This model can be used to study retinal edemamechanisms and relevant drugs.