Effects Of Long-term Molecular Hydrogen Intervention On Physiological Indexes Of Normal Rats

Protecting human organs from injury has always been the goal of medical profession,among which peroxidation,inflammation,apoptosis and other pathological changes are the main causes of chronic organ injury.Hydrogen has protective effects on many diseases such as ischemia-reperfusion injury,inflammation,trauma and metabolic diseases,and has potential application value.However,there is a lack of research on the effects of hydrogen on the normal physiological functions of the body,and the differences of the effects of different hydrogen molecules on the intervention methods are not clear.In order to evaluate the effects of two most commonly used methods of hydrogen molecule intervention(hydrogen absorption and drinking water)on physiological function of normal SD rats,the blood routine,blood biochemical indexes and intestinal flora of normal SD rats were tested.At present,the following conclusions have been drawn from the research of this subject:1.Effects of long-term hydrogen molecular intervention on immune function of normal SD ratsCompared with the Control group,hemoglobin,monocyte,neutrophil,lymphocyte and eosinophil did not change significantly in the hydrogen molecular intervention group at 2 months,while hemoglobin,monocyte and neutrophil did not change significantly at 6 months.lymphocyte and eosinophil increased significantly.Compared with the control group,lymphocyte and eosinophil in HI group increased significantly at 6 months,while there was no significant change in HRW group,but the lymphocyte and eosinophil still showed an upward trend.It can be seen that the way of hydrogen absorption has an impact on immune cells of the body,and the specific mechanism needs to be further explored.2.Effects of long-term hydrogen molecular intervention on serum biochemical parameters in normal SD ratsCompared with the control group,blood sugar(GLU)and triglyceride(TG)did not change significantly at 2 months and 6 months in the hydrogen molecule intervention group,but uric acid(UA),cholesterol(CHO),high density lipoprotein(HDL),low density lipoprotein(LDL),alanine aminotransferase(ALT),glutathione aminotransferase(AST),total bile acid(TBA),lactate dehydrogenase(LDH),creatine.kinase(CK),creatine kinase isoenzyme(CKMB)and α-hydroxybutyrate dehydrogenase(HBDH) had significant changes.However,the effects of hydrogen uptake and drinking water as different intervention methods were not exactly the same.Compared with the control group,serum glutamic oxaloacetic transaminase(AST),α-hydroxybutyrate dehydrogenase(HBDH),lactate dehydrogenase(LDH),creatine kinase(CK),creatine kinase isoenzyme(CKMB)in HI group had significant changes at 2 months and 6 months,and glutamic alanine transaminase(ALT)had significant changes.(ALT,uric acid(UA),high density lipoprotein(HDL),total bile acid(TBA)had significant changes only at 6 months;α-hydroxybutyrate dehydrogenation(HBDH),total bile acid(TBA),high density lipoprotein(HDL)had significant changes only at 2 months,serum cholesterol(CHO),at 2 months and 6 months in HRW group.3.Effects of long-term hydrogen molecular intervention on intestinal flora in normal SD ratsThrough statistical analysis of intestinal flora including phylum,class,order,family and genus,it was found that long-term hydrogen molecular intervention had a significant effect on the structure and abundance of intestinal flora in SD rats.The abundance of Firmicutes and the ratio of Bacteroidetes(F/B)decreased.Considering the correlation between body weight and lipid metabolism in SD rats,we found that long-term hydrogen molecular intervention had a significant effect on the composition and abundance of intestinal flora.The significant increase in the abundance of Prevotella may be related to the increase of blood sugar in rats.The results of this study can provide basic research data for the clinical application of hydrogen molecules,and also provide important clues for the study of the mechanism of hydrogen molecules.