Analysis And Research On The Difference Of Gene Expression Profile In Androgenetic Alopecia Patients

Objective To analyze the gene expression spectrum chip of androgenetic alopecia(AGA)and to screen different genes by bioinformatics methods,in order to analyze its relationship with the mechanism of AGA.Methods Based on the hair follicle specimens obtained from the hair transplantation of the medical institution,the RNA sequencing technology was used to preliminarily screen the candidate differential genes related to the anterior hairline(or the edge of the top alopecia area)and the pillow hair follicle.To analyze the function and pathway enrichment of differential genes through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and use the real-time fluorescence quantitative PCR(qRT-PCR)to detect the expression of target genes in the hair follicle tissues of AGA patients.Results A total of 705 differential genes corresponding to mRNA were screened.GO analysis showed that differential genes corresponding to mRNA were mainly involved in biological processes such as cellular metabolic process,intracellular membrane-bounded organelle,and binding.KEGG analysis showed that differentially expressed mRNA target genes are mainly enriched in items such as legionellosis,NOD-like receptor signaling pathway,TNF signaling pathway,Leukocyte transendothelial migration,and so on.A total of 14 genes with significant differences were screened out,and qRT-PCR was used to verify the three genes CRABP2,PSAP and NAPA.QRT-PCR showed that CRABP2、PSAP and NAPA were highly expressive in hair follicle tissue in alopecia,and the difference was statistically significant.Conclusion CRABP2,PSAP and NAPA are highly expressed in the hair follicle tissues of AGA patients.The differentially expressed mRNA target genes are significantly enriched in the NOD-like receptor signaling pathway,and they may be involved in the pathogenesis of AGA.