CYP11B2 And AT1R Gene Polymorphisms And Their Expression In Pregnancy-induced Hypertension Of Han Group In Qinghai Province

Objective: To study the aldosterone synthase(aldosterone synthase,CYP11B2)gene-344T/C polymorphism,angiotensin Ⅱtype 1 receptor(angiotensin Ⅱtype 1receptor,AT1R)gene A1166 C polymorphism and Qinghai province Han pregnancyinduced hypertension disease(hypertensive disorder complicating pregnancy,HDCP)patients(n=149)and normal pregnancy(n=155)genes in the distribution difference mRNA expression levels of CYP11B2 and AT1 R genes in the placenta and plasma of the two groups were detected.To analyze the risk factors of hypertension during pregnancy of Han nationality in Qinghai province.Methods: Blood DNA was used as template to detect CYP11B2 gene-344T/C polymorphism and AT1 R gene A1166 C polymorphism by PCR-RFLP and agarose gel electrophoresis.RT-q PCR analysis of CYP11B2 and AT1 R in placenta tissue was performed by RT-q PCR.The expression of CYP11B2 and AT1 R in plasma was detected by ELISA.The distribution of plasma CYP11B2 level in CYP11B2-344T/C polymorphism was detected.Results:1.The distribution of CYP11B2 genotype was in line with the hardy-weinberg genetic balance(df=2,refer to table 2,P > 0.05,reaching the genetic balance),and there was a difference in the distribution frequency of ACE genotype between the HDCP group and the Control group(χ~2=6.747,P<0.05).The CC genotype of the HDCP group was significantly higher than that of the control group,and the C allele frequency of the HDCP group was higher than that of the control group(χ~2=4.354,P<0.05).2.The distribution of AT1 R genotypes was in line with the hardy-weinberg genetic balance(df=2,look up table 2,P > 0.05,reaching the genetic balance),and there was a difference in the distribution frequency of AT1 R genotypes between the HDCP group and the Control group(χ~2=7.331,P<0.05).The AC genotypes in the HDCP group were significantly higher than those in the control group,and the C allele frequency in the HDCP group was higher than that in the control group(χ~2=6.958,P<0.05).3.The plasma CYP11B2 level of the HDCP group was(73.20±18.92)μg/L,Control group was(64.8±21.05)μg/L,and the CYP11B2 level of the HDCP group was significantly higher than that of the control group(P =0.000<0.05).The plasma AT1 R level of the HDCP group was(51.36±19.38)μg/L,and that of the Control group was(50.48±21.54)μg/L.The AT1 R level of the HDCP group was higher than that of the control group,but the difference was not statistically significant(P=0.101>0.05).The CYP11B2 concentration of the three genotypes was significantly different(P = 0.004 < 0.05),and the TC was significantly different from the TT genotype(P =0.048 < 0.05),and the TC was higher than the TT genotype.The difference between TC and CC was significant(P =0.001<0.05)and the CC was higher than TC.There was no significant difference between TT and CC(P =0.863 >0.05).4.Relative mRNA expression level of CYP11B2 gene in HDCP group was1.497±0.106,control group was 1.081±0.067,and HDCP group was higher than Control group(P <0.05).The relative mRNA expression level of AT1 R gene in HDCP group was1.456±0.155 and Control group was1.363±0.254,and the difference between the two groups was not statistically significant(P >0.05).Conclusion:1.CYP11B2-344T/C polymorphism and AT1 R A1166C polymorphism are related to pregnancy hypertension of the Han nationality in Qinghai province.The C allele of the two may be the susceptibility gene of HDCP;The expression of plasma CYP11B2 is related to pregnancy hypertension of the Han nationality in Qinghai province,and the CYP11B2 concentration in CYP11B2-344T/C polymorphism is CC>TC>TT,CC genotype may control the CYP11B2 level,and plasma CYP11B2 concentration has the value of diagnosing HDCP.2.The expression of CYP11B2 mRNA in placenta tissue is related to the occurrence of pregnancy-induced hypertension in Qinghai Province.