| objective The L5 and L6 spinal nerve roots of rabbits were cut by selective posterior rhizotomy(SPR)to make a knee osteoarthritis model based on proprioception.Observe the changes in electromyogram(EMG)values and the front of the rabbits in different groups.Changes in the number and morphology of proprioceptors(Bielschowsky nerve staining)in the cruciate ligament(ACL),and changes in the morphology of articular cartilage tissue(HE staining),in order to explore the mechanism of the treatment of knee osteoarthritis.method Thirty adult healthy SPF New Zealand white rabbits of 2.0-2.5 kg,male,were divided into normal group,10 model group and treatment group according to the principle of completely random allocation.After 1 week of adaptive feeding,the SPR method was used to start modeling and establish a KOA model.Grouping:(1)Normal group: regular feeding without any treatment;(2)Model group: The rabbit model of knee osteoarthritis was established by SPR method.After skin preparation,routine disinfection,and anesthesia,a longitudinal incision was made at the right side of L4-L7 at the midline of the rabbit’s spine.The L5 and L6 spinal nerve roots were cut off,hemostasis,physiological saline washing,sutured incisions layer by layer,and 200,000 injections were made.Penicillin was used to prevent infection and was routinely reared after the operation without treatment;(3)Treatment group: The modeling method was the same as that of the model group.Two months after the modeling,they were treated with the manipulation of tendons and bones.Specific method: According to the six meridians corresponding to the foot Yangming,Foot Sun,Foot Shaoyang,Foot Taiyin,Foot Jueyin and Foot Shaoyin that follow the knee joints of the human body,the anterior and posterior medial and lateral muscle groups are used for the right lower limb of the model rabbit.Manipulative therapy.First,the rabbit’s right lower limb muscles are pressed,rubbed and relaxed for 3-5 minutes,and then the cord-like tendons and nodules are treated along the meridian route.The rabbit’s popliteal fossa,quadriceps tendon and Knead the soft tissue around the knee 50 times,and then pull and extend the rabbit knee joint to achieve the purpose of traction.Each time,30 seconds,and finally flex and extend the rabbit knee joint 10 times.Each treatment was for 10 minutes,1 time/2d,and continued treatment for 21 times.Observe the Bielschowsky nerve staining to observe the number and morphological changes of proprioceptors;observe the morphological and structural changes of the cartilage stained by HE;and verify the success of the model based on this.After the model was successfully built,the KOA rabbits in the treatment group were treated with the manipulation of tendons and bones,and the rest of the groups were bred routinely without treatment.After the treatment,the following tests were performed on the three groups of rabbits:electromyography(EMG)test,gross observation and Pelletier JP score,proprioceptor observation(Bielschowsky nerve stain),cartilage morphology observation(HE stain)and modified Mankin’s score,cartilage Observation of cell apoptosis(Tunel staining).Finally,perform statistical analysis on the data.result(1)The comparison result of the incubation period and peak value of EMG: The difference between the three groups was statistically significant(P<0.05).Compared with the normal group,the incubation period of the model group is significantly longer than that of the normal group(P<0.05);compared with the treatment group,the incubation period of the treatment group is significantly lower than that of the model group(P<0.05),and the difference is statistically significant Significance: Compared with the normal group,the treatment group has a longer incubation period,which is significantly different(P<0.05).In the comparison of peaks,the model group has a significantly lower peak than the normal group than the normal group(P<0.05);compared with the treatment group,the peak of the treatment group is significantly higher than that of the model group(P<0.05).0.05),the difference is statistically significant;compared with the normal group,the treatment group has a lower wave peak,and there is a significant difference(P<0.05).(2)Gross observation of cartilage and Pelletier JP score results: the cartilage surface of the normal group was smooth and intact,the color was normal,and there was no synovial tissue hyperplasia;the model group had joint hypertrophy in the later stage,the surface of the articular cartilage cartilage was rough,and the color was dull;the cartilage of the treatment group showed It is relatively smooth,with a small amount of spot-like damage on the surface,with a general color and pale white.Compared with the normal group,the cartilage damage in the model group was more serious(P<0.05).Compared with the treatment group,the cartilage damage in the model group was more severe(P<0.05).Compared with the normal group,the treatment group Cartilage damage was obvious,and the results were statistically different(P<0.05).(3)Bielschowsky nerve staining proprioceptor count results: Compared with the normal group,the number of proprioceptors in the model group was significantly reduced(P<0.05).Compared with the treatment group,the number of proprioceptors in the treatment group was significantly more than that in the model group.,There was a statistical difference in the results(P<0.05);compared with the normal group,the number of proprioceptors in rabbit ACL decreased in the treatment group,and there was a difference(P<0.05).(4)Cartilage tissue observation and modified Mankin’s score results: the cartilage surface structure of the normal group is smooth and complete,the inner cartilage layer is clear,cartilage cells are not aggregated,cartilage cells are normal in shape,and the tide line does not disappear.Model group: Cartilage structure is damaged,cracked,cartilage levels are disordered,cartilage cells are not arranged in a columnar shape,cells are aggregated,and the tide line disappears;Manipulation group: The cartilage shows a relatively smooth structure without damage,and the cartilage levels are basically clear.The cells near the cartilage surface have less aggregation,and the cell morphology and structure are basically normal.Among the three groups,the difference was statistically significant(P<0.01).Compared with the normal group,the cartilage damage in the model group is more serious(P<0.05);compared with the treatment group,the rabbit cartilage damage in the model group is more severe(P<0.05).Compared with the normal group,the treatment group is There were statistical differences in the results of articular cartilage scores(P<0.05).(5)Comparison of the results of chondrocyte apoptosis: Compared with the normal group,the number of chondrocyte apoptosis in the model group was reduced(P<0.05);compared with the treatment group,the number of chondrocyte apoptosis in the model group was more than that in the treatment group(P<0.05);Comparing the treatment group with the normal group,the number of chondrocyte apoptosis in the normal group was more than that in the normal group(P<0.05),and the result was statistically significant(P<0.05).conclusion(1)The manipulation of tendons and bones can reduce the damage of proprioceptors in the soft tissues,thereby strengthening the proprioceptive function of the knee joint,and achieve the purpose of maintaining the mechanical stability of the knee joint.(2)The manipulation of tendons and bones can maintain the mechanical stability of the knee joint to alleviate the degree of cartilage damage,reduce the abnormal apoptosis of cartilage cells,and maintain the relative integrity of the cartilage tissue to achieve the purpose of protecting the knee joint. |
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