| ObjectiveIn this study,insomnia model rats prepared by intraperitoneal injection of para-chlorophenylalanine were selected as the research object,and "Baihui" and "Shenshu" were selected for Biao-ben with acupuncture point,to observe the general state,behavior,content of neurotransmitters Glu and GABA,ratio of Glu/GABA in the hypothalamus,and expression levels of GAD and GS proteins.To explore the effectiveness and possible mechanism of regulating Glu and GABA in the treatment of insomnia by " Biao-ben acupoints" acupuncture,and to provide experimental basis for the treatment of insomnia by " Biao-ben acupoints" acupuncture.MethodForty-eight healthy male three-month-old SD rats with body weight of about（160-180）g were randomly divided into no-treatment control group,model group,Biao-ben acupoints group and non-acupoint group,with 12 rats in each group.The rat model of insomnia was made by intraperitoneal injection of PCPA for two consecutive days.After successful modeling,acupuncture intervention was initiated.No-treatment control group and model group did not do any intervention;In the Biao-ben acupoints group,Baihui and Shenshu were given acupuncture stimulation,Baihui by the supplementation method and Shenshu by the draining method.The acupuncture was performed once every five minutes,one minute each time,and the needle was retained for twenty minutes,once a day,for a total of seven days.Non-acupoint group was given acupuncture stimulation at 0.3cm non-acupoint beside Baihui and Shenshu,and the needle was retained for twenty minutes,once a day,for consecutive seven days.The general state and behavioral changes of rats were observed,the content of GABA and Glu,the ratio of Glu/GABA and the expression levels of GAD and GS proteins in the hypothalamus of rats were recorded after intervention,and the data of each group were statistically analyzed Result1.Comparison of general state: After modeling,except no-treatment control group,the other three groups of rats had poor mental state,hair became dry,dull,easy to fall off,increased activity and easy to anger,abnormal sensitivity to external stimulation such as sound and light,and aggressive behavior was enhanced.After the intervention,the spirit of the rats in the Biao-ben acupoints group improved,with regular hair and reduced aggression.The excitability of the model group and the nonacupoint group decreased significantly,showing weak and dispirited,preferring static and less movement.2.Behavioral comparison: Comparison among groups: After modeling,compared with the no-treatment control group,the experimental scores of model group,Biao-ben acupoints group and non-acupoint group were significantly increased（P<0.05）;After the intervention,compared with the no-treatment control group,the experimental scores of the model group and the non-acupoint group were significantly decreased（P<0.05）,while the difference was not significant in the Biao-ben acupoints group（P>0.05）.Compared with the model group,the experimental scores of the Biao-ben acupoints group and the non-acupoint group were increased,and there was a significant difference in the Biao-ben acupoints group（P<0.05）,but no significant difference in the non-acupoint group（P>0.05）.Compared with the non-acupoint group,the experimental scores of rats in the Biao-ben acupoints group were higher（P<0.05）.Comparison within groups: Compared with before modeling,the experimental scores of rats in model group,Biao-ben acupoints group and non-acupoint group increased after modeling（P<0.05）,and the experimental scores of rats in the model group and the non-acupoint group significantly decreased after intervention（P<0.05）;Compared with after modeling,the experimental scores of the Compared with before modeling,the experimental scores of rats in model group,Biao-ben acupoints group and non-acupoint group after intervention were significantly decreased（P<0.05）.3.Comparison of GABA and Glu contents and Glu/GABA ratio:Compared with the no-treatment control group,Glu content in model group was significantly increased（P<0.01）,GABA content was decreased（P<0.01）,Glu/GABA ratio was significantly increased（P<0.01）,while Glu content in non-acupoint group was not significantly different（P>0.05）,GABA content was decreased（P<0.01）,the ratio of Glu/GABA was significantly increased（P<0.01）,and there was no significant difference between the Biao-ben acupoints group and the no-treatment control group（P>0.05）;Compared with the model group,Glu content in the Biao-ben acupoints group was decreased（P<0.05）,GABA content was increased（P<0.05）,and Glu/GABA ratio was decreased（P<0.05）,and the non-acupoint group Glu content had no significant difference（P>0.05）,GABA content was increased（P<0.05）,Glu/GABA ratios was decreased（P<0.05）;Compared with the non-acupoint group,the content of Glu in the Biao-ben acupoints group was not significantly different（P>0.05）,the content of GABA was higher（P<0.01）,and the ratio of Glu/GABA was lower（P<0.01）.4.Comparison of GAD and GS protein expression levels: Compared with the no-treatment control group,the expression of GAD in the hypothalamus of model group was significantly decreased（P<0.05）,while the expression of GS was significantly increased（P<0.05）,and there was no statistical difference between the Biao-ben acupoints group（P>0.05）,the expression of GAD in non-acupoint group was not significantly different（P>0.05）,but the expression of GS was increased（P<0.05）;Compared with the model group,the expression of GAD was increased（P<0.05）and the expression of GS was decreased（P<0.05）in the Biao-ben acupoints group,while GAD expression was increased（P<0.05）in the non-acupoint group,but there was no significant difference in GS expression（P>0.05）;Compared with the non-acupoint group,the expression of GAD was not significantly different in the Biao-ben acupoints group（P>0.05）,but the expression of GS was lower（P<0.05）.ConclusionsAcupuncture with “Combined Biao and Ben acupoints” can improve the general state and behavioral symptoms of PCPA insomnia rats,and regulate the metabolic balance between excitatory Glu and inhibitory GABA by up-regulating the expression of GAD protein and down-regulating the expression of GS protein. |
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