| Objective:Through clinical verification of the curative effect of Qigong Huatan prescription in the treatment of phlegm-dampening polycystic ovary syndrome,the network diagram of Qigong Huatan prescription-active ingredient-core gene-signal pathway-polycystic ovary syndrome was constructed by using the method of network pharmacology study,and then the mechanism of its treatment was discussed.It provides a new concept and research strategy for the study of clinical efficacy and mechanism of Qigong Huatan Prescription in the treatment of polycystic ovary syndrome.Methods:1.Clinical studies: 100 patients meeting inclusion criteria were selected and divided into treatment group(n=50)and control group(n=50)by random number table method.The treatment group was treated with Qigong Huatan Prescription,and the control group was treated with ethanestradiol cyproterone tablets.Both groups were treated for 3 consecutive cycles,each cycle was 28 days.The changes of serum sex hormone level,BMI value,acne,menstrual cycle and TCM syndrome score before and after treatment were observed,and the curative effect was analyzed.The menstrual cycle was followed up 3 months after drug withdrawal.2.Network Pharmacology Studies:The chemical components and drug targets of Qigong Huatan Prescription were screened by TCMSP,and the disease targets of POLYcystic ovary syndrome were obtained by Genecards,Pharm Gkb,OMIM and Drugbank databases,and the core genes of both were found.Mapping the intersection target protein-protein interaction(PPI)network;Through GO enrichment analysis and KEGG pathway analysis of therapeutic targets,Cytoscape software was used to draw the interaction network diagram of Qigong Huatan prescription-active component-core gene-signal pathway-POLYcystic ovary syndrome.Results:1.After statistical treatment of clinical efficacy:(1)Comparison of general data: the two groups were comparable in age,height and weight before treatment(P>0.05).(2)Comparison of serum sex hormones: compared with the two groups before treatment,the improvement of FSH,LH,LH/FSH and T was effective,with statistical significance(P<0.05).The comparison of T,LH and LH/FSH ratio between the two groups was statistically significant(P<0.05),and the curative effect of the treatment group was inferior to the control group.There was no significant difference between the FSH groups and the FSH groups(P>0.05).(3)Comparison of BMI values: There were differences in BMI values between the two groups before and after drug withdrawal(P<0.05);There was no significant difference between the two groups when the drug was stopped(P>0.05).However,the decrease of BMI in the treatment group was more significant than that in the control group,and the difference between the two groups before and after drug withdrawal was statistically significant(P<0.05).(4)Comparison of acne scores: There were statistically significant differences between the two groups of acne scores before and after drug withdrawal(P<0.05),suggesting that both treatment regimens could improve patients’ acne.There was no significant difference between the two groups when the drug was stopped(P>0.05).However,the improvement effect was more obvious in the control group,and the difference between the two groups when the drug was stopped and before treatment was statistically significant(P<0.05).(5)Comparison of TCM syndromes: In terms of improving TCM syndromes,TCM syndrome scores of patients in the two groups decreased when drug withdrawal and before treatment,with statistical significance(P<0.05);The improvement of TCM syndrome in treatment group was significantly higher than that in control group,and the difference was statistically significant(P<0.05).(6)Comparison of menstrual cycle: compared with the two groups before treatment,menstrual cycle could be improved in both groups,with statistical significance(P<0.05);The improvement of menstrual cycle was more obvious in the control group,and the difference was statistically significant(P<0.05).There was no significant difference between the treatment group 3months after drug withdrawal and the treatment group at the time of drug withdrawal(P>0.05),and the menstrual cycle control was relatively stable.However,there was statistically significant difference between the control group 3 months after drug withdrawal and the control group at the time of drug withdrawal(P<0.05),suggesting poor control of menstrual cycle 3months after drug withdrawal.2.Using the method of network pharmacology research,Qigong Huatan Prescription according to the screening of 82 active ingredients and 365 predicted targets,disease targets 1483;134 core genes were screened,and2598 biological process items,84 cell component items and 200 molecular function items were obtained by GO enrichment analysis.KEGG pathway analysis yielded 173 entries.Conclusion(s):1.After clinical studies:(1)Qigong Huatan prescription is inferior to ethanestradiol cyproterone in improving serum sex hormone level.The efficacy of ethinylestradiol cyproterone tablets in improving acne is equivalent to that of ethinylestradiol cyproterone tablets.Compared with ethinylestradiol cyproterone tablets,the treatment effect was significantly better in improving BMI.(2)Qigong Huatan Prescription is superior to ethanestradiol cyproterone tablet in improving TCM syndrome;In addition,according to the follow-up 3months after drug withdrawal,Qigong Huatan Prescription has better long-term efficacy in improving menstrual cycle.2.Through network pharmacology analysis,Qigong Huatan Prescription mainly treated polycystic ovary syndrome through its active ingredients quercetin,kaempferol,hanbaicalein,β-sitosterol,etc.Pi3k-akt,age-rage,MAPK and other signaling pathways can be mediated by HIF1 A,MAPK3,IL6,MMP9,JUN,PTGS2 and other core genes. |
PDF Full Text Request