Characteristics Of Intestinal Microflora In Patients With Liver Depression Syndrome In Prediabetes Mellitus

Objective:To study the distribution and abundance characteristics of intestinal flora in PATIENTS with PDM liver depression syndrome,compared with patients with PDM non-liver depression syndrome and T2 DM liver depression syndrome,the healthy group was the control group,and compared their differences,so as to provide theoretical basis for TCM treatment of PDM patients with intestinal flora administration and provide biological research basis for in-depth study of PDM and liver depression syndrome.Methods:1.Patients meeting the requirements of this project were collected from the Third People’s Hospital affiliated to Fujian University of Chinese Medicine,Shishi General Hospital and Shishi Hubin Community Health Service Center;The patients were divided into four groups: PDM liver depression syndrome group,PDM non-liver depression syndrome group,T2 DM liver depression syndrome group and healthy group for comparison and analysis.2.Screening for impaired fasting blood glucose/abnormal glucose tolerance(including fasting blood glucose,2-hour postprandial blood glucose,hba1 c,and glucose tolerance test;If one index was abnormal,the patient was identified as PDM patient),and the patient’s four-diagnosis information and basic information(including gender,age,height,BMI index,weight,waist circumference,etc.)were collected.3.Fecal samples of patients meeting inclusion criteria were collected for 16 S r DNA gene sequencing,and the distribution and abundance of intestinal flora were analyzed,and the differences of bacterial genera between groups were compared and analyzed.Results:1.The waist circumference of PDM group without liver depression syndrome was significantly different from that of healthy group(P < 0.01);There were significant differences in body weight and BMI between T2 DM group and healthy group(P < 0.05).2.The difference between groups was greater than that within groups,and the difference between T2 DM liver depression syndrome group and the other three groups was significant(P=0.00061).There were differences in the composition of intestinal flora genera between PDM liver depression syndrome group,PDM non-liver depression syndrome group,T2 DM liver depression syndrome group and healthy group.Among them,the top ten levels of intestinal flora in PDM Liver depression syndrome group were: Bacteroides,Prevotella,Faecalibacterium,Megamonas,Phascolarctobacterium,Escherichia-shi Gella,Parabacteroides,Fusobacterium,Lachnoclostridium,Lachnospira.The top ten levels of intestinal flora in PDM group without Liver depression syndrome were: Bacteroides,Megamonas,Prevotella,Fusobacterium,Faecalibacterium,Phascolarctobacterium Escherichia-shigella,Parabacteroides,Lachnospiraceae,Lachnoclostridium.The top ten levels of intestinal flora in T2 DM liver depression syndrome group were: Bacteroides,Megamonas,Prevotella,Escherichia-Shigella,Fusobacterium,Phascolarctobacte Rium,Faecalibacterium,Lachnoclostridium,Parabacteroides,Lachnospiraceae.3.Compared with the non-PDM group,the differences of Prevotella,Faecalibacterium and Lachnoclostridium were statistically significant,and the abundance of Prevotella was higher in PDM group.The difference of Megamonas was statistically significant,and the abundance of Megamonas was lower in PDM liver depression syndrome group.There were significant differences in Bacteroides,Prevotella,Faecalibacterium and Roseburia between PDM group and T2 DM group,and the abundance of PDM group was higher.Megamonas(Megomonas),Phascolarctobacterium(koala),Escherichia-Shigella(Escherichia)and Lachnoclostridium had statistically significant differences,while the abundance of PDM liver depression syndrome group was lower.T2 DM group with liver depression syndrome and PDM group without liver depression syndrome were compared.Phascolarctobacterium(Koala),Escherichia-Shigella,Lachnospiraceae,Lachnoclostridium,Agathobacter The difference was statistically significant,and the abundance was higher in T2 DM group with liver depression syndrome.Bacteroides(Bacteroides),Prevotella(Prevotella),Faecalibacterium(Fafaella),Megamonas(giant monocell bacteria)and Roseburia(Roche)showed statistically significant differences,while the abundance of T2 DM liver depression syndrome group was lower.The differences of Megamonas(Megomonas),Phascolarctobacterium(koala)and Lachnoclostridium between PDM liver depression syndrome group and healthy group were statistically significant,and the abundance of PDM liver depression syndrome group was higher.The differences of Prevotella,Faecalibacterium,Roseburia and Agathobacter were statistically significant,and the abundance of PDM liver-depression syndrome group was lower.The differences of Megamonas,Koala,Escherichia-Shigella,Lachnospiraceae and Lachnoclostridium between T2 DM liver depression syndrome group and healthy group were statistically significant.The abundance of T2 DM patients with liver depression syndrome was higher.Bacteroides,Prevotella,Faecalibacterium,Roseburia and Agathobacter had statistical significance,and the abundance of T2 DM liver depression syndrome group was lower.Conclusion:1.Liver depression syndrome may be one of the factors affecting body weight,waist circumference,etc.2.There were differences in the composition of genus level abundance of intestinal flora among PDM liver depression syndrome group,PDM non-liver depression syndrome group,T2 DM liver depression syndrome group and healthy group.The composition of giant unicellular bacteria in PDM patients with liver depression syndrome was lower than that in non-liver depression syndrome group.The composition of Bacteroides,Prevotella and Roche in T2 DM patients with liver depression syndrome was lower than that in PDM patients without liver depression syndrome.Therefore,the probiotics composition ratio of PDM and T2 DM intestinal flora is different,and there may be a gradual evolution process between them.So,the changes in the composition of these bacteria may be related to "liver depression syndrome".3.Probiotics may improve IGT patients with liver depression syndrome;It can provide theoretical basis for clinical prevention and treatment of PDM and liver depression syndrome.